Identification of six cell surface proteins for specific liver targeting. Issue 7 (August 2015)

• Record Type: Journal Article
• Authors: Ducret, Axel; Kux van Geijtenbeek, Sabine; Röder, Daniel; Simon, Sandrine; Chin, Daniel; Berrera, Marco; Gruenbaum, Lore; Ji, Changhua; Cutler, Paul; Cutler, Paul; Voshol, Hans
• Is Part Of: Proteomics. Volume 9:Issue 7/8(2015)
• Page Start: 651
• Page End: 661
• Publication Date: 2015-08
• Subjects: Proteomics -- Periodicals; 572.605
• DOI: 10.1002/prca.201400194
• Languages: English
• ISSNs: 1862-8346

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="prca1684-sec-0010" sec-type="section"> <title>Purpose</title> <p>Cell surface proteins are the primary means for a cell to sense and interact with its environment and their dysregulation has been linked to numerous diseases. In particular, the identification of proteins specific to a single tissue type or to a given disease phenotype may enable the characterization of novel therapeutic targets. We tested here the feasibility of a cell surface proteomics approach to identify pertinent markers directly in a clinically relevant tissue.</p> </sec> <sec id="prca1684-sec-0020" sec-type="section"> <title>Experimental design</title> <p>We analyzed the cell surface proteome of freshly isolated primary heptatocytes using a glycocapture‐specific approach combined with a robust bioinformatics filtering.</p> </sec> <sec id="prca1684-sec-0030" sec-type="section"> <title>Results</title> <p>Using primary lung epithelial cell cultures as negative controls, we identified 32 hepatocyte‐specific cell surface proteins candidates. We used mRNA expression to select six markers that may provide adequate specificity for targeting therapeutics to the liver.</p> </sec> <sec id="prca1684-sec-0040" sec-type="section"> <title>Conclusions and clinical relevance</title> <p>We demonstrate the feasibility and the importance of conducting such studies directly in a clinically relevant tissue. In particular, the cell surface proteome of freshly isolated hepatocytes differed substantially from cultured cell lines.</p> </sec> </abstract>