Microphthalmia transcription factor immunohistochemistry for FNA biopsy of ocular malignant melanoma. Issue 7 (24th February 2015)

• Record Type: Journal Article
• Authors: Perrino, Carmen M.; Wang, Jeff F.; Collins, Brian T.
• Is Part Of: Cancer cytopathology. Volume 123:Issue 7(2015:Jul.)
• Page Start: 394
• Page End: 400
• Publication Date: 2015-02-24
• Subjects: Cancer -- Cytopathology -- Periodicals; Pathology, Cellular -- Periodicals; Cytology -- Technique -- Periodicals; 611.01815
• DOI: 10.1002/cncy.21531
• Languages: English
• ISSNs: 1934-662X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="cncy21531-sec-0001" sec-type="section"> <title>BACKGROUND</title> <p>Microphthalmia transcription factor (MiTF) is a sensitive and specific immunohistochemistry (IHC) marker for malignant melanoma (MM) in surgical resections, but its utility in cytology specimens has not been extensively studied.</p> </sec> <sec id="cncy21531-sec-0002" sec-type="section"> <title>METHODS</title> <p>A search of the pathology database identified fine‐needle aspiration biopsies signed out as MM or suspicious for MM (1998‐2012). The final diagnosis and the IHC results were recorded, and all slides were re‐reviewed. MiTF IHC (C5/D5 cocktail) was performed on alcohol‐fixed, Papanicolaou‐stained direct smears. Any amount of nuclear staining was considered positive. Staining was scored on a scale of 1 to 4 (1, 1%‐24%; 2, 25%‐49%; 3, 50%‐74%; 4, 75%‐100%).</p> </sec> <sec id="cncy21531-sec-0003" sec-type="section"> <title>RESULTS</title> <p>Two hundred seventy‐four cases were diagnosed as MM, and 24 were diagnosed as suspicious for MM. IHC staining was performed for 25% (69 of 274) of the MM cases and for 33% (8 of 24) of the cases suspicious for MM. MiTF IHC of Papanicolaou slides was performed for 37% (101 of 274) of the MM cases (positive, 82% [83 of 101]; negative, 4% [4 of 101]; noncontributory, 14% [14 of 101]) and for 33% (8 of 24) of the cases suspicious for MM (positive, 38% [3 of 8]; noncontributory, 62% [5 of 8]). The majority of positive staining was scored as 3 or 4. The sensitivity of MiTF on Papanicolaou‐stained slides was 95%.</p> </sec> <sec id="cncy21531-sec-0004" sec-type="section"> <title>CONCLUSIONS</title> <p>MiTF is a useful IHC stain for the evaluation of ocular MM, and it is effective when it is performed on alcohol‐fixed, Papanicolaou‐stained aspirate smears. <bold><italic>Cancer (Cancer Cytopathol)</italic> 2015;123:394‐400.</bold> © <italic>2015 American Cancer Society.</italic></p> </sec> </abstract>