Evaluation of the Etest method for detecting colistin susceptibility of multidrug-resistant Gram-negative isolates in Vietnam. Issue 8 (August 2015)

• Record Type: Journal Article
• Authors: Nhung, Pham Hong; Miyoshi-Akiyama, Tohru; Phuong, Doan Mai; Shimada, Kayo; Anh, Nguyen Quoc; Binh, Nguyen Gia; Thanh, Do Van; Ohmagari, Norio; Kirikae, Teruo
• Is Part Of: Journal of infection and chemotherapy. Volume 21:Issue 8(2015:Aug.)
• Page Start: 617
• Page End: 619
• Publication Date: 2015-08
• Subjects: Chemotherapy -- Periodicals; Infection -- Periodicals; Communicable diseases -- Chemotherapy -- Periodicals; 615.5805
• DOI: 10.1016/j.jiac.2015.04.002
• Languages: English
• ISSNs: 1341-321X

Abstract

<abstract xml:lang="en" abstract-type="author" id="abs0010"> <title id="sectitle0010">Abstract</title> <sec> <p id="abspara0010">The minimum inhibitory concentrations (MICs) of colistin for 241 multidrug-resistant (MDR) Gram-negative pathogens were determined by the Etest and by the broth microdilution method (BMD). The two methods showed essential agreements of 76% (77/102) for <italic>Acinetobacter baumannii</italic>, 90% (36/40) for <italic>Pseudomonas aeruginosa</italic> and 84% (83/99) for <italic>Enterobacteriaceae</italic> isolates, with categorical agreements of 100%, 98%, and 100%, respectively. Of the 241 isolates, none showed a very major error and one (0.4%) showed a major error. MICs ranged from 0.125 to 0.5 μg/ml for all <italic>A. baumannii</italic> and most <italic>Enterobacteriaceae</italic> isolates, and from 1 to 2 μg/ml for most <italic>P. aeruginosa</italic> isolates. Of the 40 <italic>P. aeruginosa</italic> isolates, 27 (68%) showed higher colistin MICs by the Etest than by the BMD. In contrast, 77% (78/102) of the <italic>A. baumannii</italic> and 57% (56/99) of the <italic>Enterobacteriaceae</italic> isolates showed lower colistin MICs by the Etest than by the BMD. The Etest is a reliable and easy-to-use method to measure colistin MICs of MDR Gram-negative pathogens in clinical laboratories and can be used following validation by microdilution methods.</p> </sec> </abstract>