Experimental liver protection of n-butanolic extract of Astragalus monspessulanus L. on carbon tetrachloride model of toxicity in rat. (July 2015)

• Record Type: Journal Article
• Authors: Simeonova, Rumyana; Bratkov, Viktor M.; Kondeva-Burdina, Magdalena; Vitcheva, Vessela; Manov, Vassil; Krasteva, Ilina
• Is Part Of: Redox report. Volume 20:Number 4(2015)
• Page Start: 145
• Page End: 153
• Publication Date: 2015-07
• Subjects: Free radicals (Chemistry) -- Pathophysiology -- Periodicals; Oxidation, Physiological -- Periodicals; 541.224
• DOI: 10.1179/1351000214Y.0000000115
• Languages: German
• ISSNs: 1351-0002

Abstract

<abstract> <title> <x content-type="archive" xml:space="preserve">Abstract</x> </title> <sec> <title>Objective</title> <p>To investigate the hepatoprotective potential of <italic>n</italic>-butanolic extract of <italic>Astragalus monspessulanus</italic> L. (EAM) against <italic>in-vitro/in-vivo</italic> carbon tetrachloride (CCl<sub>4</sub>)-induced liver damage in rats. Silymarin was used as a positive control.</p> </sec> <sec> <title>Methods and results</title> <p>The <italic>in-vitro</italic> experiments were carried out in primary isolated rat hepatocytes first incubated with CCl<sub>4</sub> (86 µmol/l). Hepatic injury was discerned by a decrease in cell viability and cell glutathione (GSH) levels, an increase in lactate dehydrogenase leakage into the medium, and an elevation in malondialdehyde (MDA) quantity. Cell pre-incubation with EAM (1 µg/ml and 10 µg/ml) significantly ameliorated the CCl<sub>4</sub>-induced liver damage. <italic>In-vivo</italic> rats were challenged orally with CCl<sub>4</sub> (10% solution in olive oil) alone and after 7 days pre-treatment with EAM (100 mg/kg body weight per day, oral gavage). CCl<sub>4</sub> damage was judged by an increased production of MDA, depletion of cell GSH, and a decrease in cell antioxidant defense system. EAM pre-treatment normalizes the activities of the antioxidant enzymes and the levels of GSH and MDA. These data are supported by the histopathological examination.</p> </sec> <sec> <title>Conclusion</title> <p>These results indicate that EAM has a similar significant protective effect, <italic>in vitro</italic> and <italic>in vivo</italic>, against CCl<sub>4</sub> induced hepatotoxicity in rat as silymarin.This may be due to its antioxidant and membrane stabilizing properties.</p> </sec> </abstract>