A New Fluorescence Turn‐On Probe for Aluminum(III) with High Selectivity and Sensitivity, and its Application to Bioimaging. Issue 3 (19th February 2015)

• Record Type: Journal Article
• Authors: Jiang, Yi; Sun, Lei‐Li; Ren, Guang‐Zong; Niu, Xiang; Hu, Wen‐zhou; Hu, Zhi‐Qiang
• Is Part Of: ChemistryOpen. Volume 4:Issue 3(2015:Jun.)
• Page Start: 378
• Page End: 382
• Publication Date: 2015-02-19
• Subjects: Chemistry -- Periodicals; 540; 540.5
• DOI: 10.1002/open.201402169
• Languages: English
• ISSNs: 2191-1363

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>The development of novel selective probes with high sensitivity for the detection of Al<sup>3+</sup> is widely considered an important research goal due to the importance of such probes in medicine, living systems and the environment. Here, we describe a new fluorescent probe, <italic>N</italic>′‐(4‐diethylamino‐2‐hydroxybenzylidene)‐2‐hydroxybenzohydrazide (<bold>1</bold>), for Al<sup>3+</sup>. Probe <bold>1</bold> was evaluated in a solution of acetonitrile/water (1:1 <italic>v</italic>/<italic>v</italic>). Compared with previously reported probes for Al<sup>3+</sup>, probe <bold>1</bold> can be synthesized easily and in high yield. A Job plot confirmed that probe <bold>1</bold> is able to complex Al<sup>3+</sup> in a 1:1 ratio, and the binding constant was determined to be 4.25×10<sup>8</sup> <sc>m</sc><sup>−1</sup>. Moreover, the detection limit was as low as 6.7×10<sup>−9</sup> <sc>m</sc>, suggesting that probe <bold>1</bold> has a high sensitivity. Common coexistent metal ions, such as K<sup>+</sup>, Co<sup>2+</sup>, Ca<sup>2+</sup>, Ba<sup>2+</sup>, Ni<sup>2+</sup>, Pb<sup>2+</sup>, Hg<sup>2+</sup>, Ce<sup>2+</sup>, Zn<sup>2+</sup>, Cd<sup>2+</sup>, Fe<sup>3+</sup>, showed little or no interference in the detection of Al<sup>3+</sup> in solution, demonstrating the high selectivity of the probe. Finally, the ability of probe <bold>1</bold> to act as a fluorescent probe for Al<sup>3+</sup> in living systems was evaluated in Gram‐negative bacteria, <italic>Escherichia coli</italic>, and confocal laser scanning microscopy confirmed its utility. The results of this study suggest that <bold>1</bold> has appropriate properties to be developed for application as a fluorescent probe of Al<sup>3+</sup> for use in biological studies.</p> </abstract>