Effects of dietary Bacillus cereus G19, B. cereus BC-01, and Paracoccus marcusii DB11 supplementation on the growth, immune response, and expression of immune-related genes in coelomocytes and intestine of the sea cucumber (Apostichopus japonicus Selenka). Issue 2 (August 2015)

• Record Type: Journal Article
• Authors: Yang, Gang; Tian, Xiangli; Dong, Shuanglin; Peng, Mo; Wang, Dongdong
• Is Part Of: Fish & shellfish immunology. Volume 45:Issue 2(2015:Aug.)
• Page Start: 800
• Page End: 807
• Publication Date: 2015-08
• Subjects: Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2015.05.032
• Languages: English
• ISSNs: 1050-4648

Abstract

<abstract xml:lang="en" abstract-type="author" id="abs0010"> <title id="sectitle0010">Abstract</title> <sec> <p id="abspara0010">Probiotics have positive effects on the nutrient digestibility and absorption, immune responses, and growth of aquatic animals, including the sea cucumber (<italic>Apostichopus japonicus</italic> Selenka). A 60-day feeding trial was conducted to evaluate the effects of <italic>Bacillus cereus</italic> G19, <italic>B. cereus</italic> BC-01 and <italic>Paracoccus marcusii</italic> DB11 supplementation on the growth, immune response, and expression level of four immune-related genes (<italic>Aj</italic>-p105, <italic>Aj</italic>-p50, <italic>Aj</italic>-rel, and <italic>Aj</italic>-lys) in coelomocytes and the intestine of juvenile sea cucumbers. One group was fed the basal diet (control group), while three other groups were fed the basal diet supplemented with <italic>B. cereus</italic> G19 (G19 group), <italic>B. cereus</italic> BC-01 (BC group), or <italic>P. marcusii</italic> DB11 (PM group). The growth rate of sea cucumbers fed diets with probiotics supplementation was significantly higher than that of the control group (<italic>P</italic> < 0.05). Sea cucumbers in the G19 and PM groups had a significantly greater phagocytic activity of coelomocytes compared to the control group (<italic>P</italic> < 0.05), while those in the G19 and BC groups had a greater respiratory burst activity (<italic>P</italic> < 0.05). The alkaline phosphatase (AKP) activity of coelomocytes in sea cucumbers fed diets with probiotics supplementation was significantly higher than the control group (<italic>P</italic> < 0.05). Comparatively, superoxide dismutase (SOD) activity of coelomocytes for sea cucumber in the PM group was significantly greater (<italic>P</italic> < 0.05). As for the immune-related genes, <italic>B. cereus</italic> G19 supplementation significantly increased the expression level of the <italic>Aj</italic>-rel gene in coelomocytes (<italic>P</italic> < 0.05), while <italic>B. cereus</italic> BC-01 supplementation significantly increased that of the <italic>Aj</italic>-p50 gene as compared to the control group (<italic>P</italic> < 0.05). In the intestine, the relative expression level of <italic>Aj</italic>-p105, <italic>Aj</italic>-p50, and <italic>Aj</italic>-lys genes in the PM group was significantly higher than that in the control group (<italic>P</italic> < 0.05). These results suggested that <italic>B. cereus</italic> G19 and <italic>B. cereus</italic> BC-01 supplementation could improve the growth performance and the immune response in coelomocytes, while <italic>P. marcusii</italic> DB11 supplementation could have a positive effect on the growth performance and immune response in coelomocytes and the intestine of sea cucumbers.</p> </sec> </abstract>