In vitro and in vivo Bone‐Forming Activity of Saururus chinensis Extract. (14th April 2015)

• Record Type: Journal Article
• Authors: Moon, Seong‐Hee; Choi, Sik‐Won; Park, Sang‐Joon; Ryu, Shi‐Yong; Hwang, Kyu‐Seok; Kim, Cheol‐Hee; Kim, Seong Hwan
• Is Part Of: Phytotherapy research. Volume 29:Number 7(2015:Jul.)
• Page Start: 1073
• Page End: 1080
• Publication Date: 2015-04-14
• Subjects: Materia medica, Vegetable -- Periodicals; Botany, Medical -- Periodicals; Medicinal plants -- Periodicals; Plant Extracts -- therapeutic use -- Periodicals; Plants, Medicinal -- Periodicals; 581.634
• DOI: 10.1002/ptr.5349
• Languages: English
• ISSNs: 0951-418X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Bone is maintained by osteoclast‐mediated resorption and osteoblast‐mediated formation. Recently, anti‐osteoporotic activity of <italic>Saururus chinensis</italic> extract (SCE) and anti‐osteoclastogenic activity of its components have been reported, but the effect of SCE on bone formation has not been studied well. Therefore, in this study, we investigated whether <italic>Saururus chinensis</italic> SCE exhibits <italic>in vitro</italic> osteogenic and <italic>in vivo</italic> bone‐forming activity. extract strongly enhanced the bone morphogenetic protein (BMP)‐2‐stimulated induction of alkaline phosphatase, an early phase biomarker of osteoblast differentiation, in bi‐potential mesenchymal progenitor C2C12 cells. <italic>In vitro</italic> osteogenic activity of SCE was accompanied by enhanced expression of BMP‐2, BMP‐4, BMP‐7 and BMP‐9 mRNA. In addition, a pharmacological inhibition study suggested the involvement of p38 activation in the osteogenic action of SCE. Moreover, the BMP dependency and the involvement of p38 activation in the osteogenic action of SCE were confirmed by the treatment of noggin, an antagonist of BMP. <italic>Saururus chinensis</italic> extract also exhibited to induce runt‐related transcription factor 2 activation at the high concentration. Furthermore, the <italic>in vivo</italic> osteogenic activity of SCE was confirmed in zebrafish and mouse calvarial bone formation models, suggesting the possibility of its use for bone formation. In conclusion, we suggested that <italic>in vivo</italic> anti‐osteoporotic activity of SCE could be because of its dual action in bone, anti‐osteoclastogenic and anabolic activity. Copyright © 2015 John Wiley & Sons, Ltd.</p> </abstract>