Optimized hydrolysis and analysis of Radix Asparagi polysaccharide monosaccharide composition by capillary zone electrophoresis. Issue 13 (15th May 2015)

• Record Type: Journal Article
• Authors: Chen, Jiye; Yang, Feifei; Guo, Huaizhong; Wu, Fang; Wang, Xiaohuan
• Is Part Of: Journal of separation science. Volume 38:Issue 13(2015:Jul.)
• Page Start: 2327
• Page End: 2331
• Publication Date: 2015-05-15
• Subjects: Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201500120
• Languages: English
• ISSNs: 1615-9306

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Using orthogonal design, optimized conditions for the hydrolysis of the polysaccharide from <italic>Radix Asparagi</italic> were determined, as well as its monosaccharide composition. Optimized hydrolysis conditions were a temperature of 100°C in 1.5 M sulfuric acid solution for 5 h. The resulting monosaccharides were derivatized with 1‐phenyl‐3‐methyl‐5‐pyrazolone, then separated by capillary zone electrophoresis in 40 mM sodium tetraborate buffer (pH 10.1), and detected by ultraviolet absorption at 245 nm. Results indicate that the polysaccharide from <italic>Radix Asparagi</italic> is composed of xylose, arabinose, glucose, rhamnose, mannose, galactose, glucuronic acid, and galacturonic acid, which differs from published findings. Moreover, xylose, glucuronic acid, and galacturonic acid have not been previously reported in <italic>Radix Asparagi</italic> polysaccharide. This method is simple, fast, and yields a highly efficient separation. As well, these findings can be applied to quality control of <italic>Radix Asparagi</italic> and for in‐depth study of the biological activity of <italic>Radix Asparagi</italic> polysaccharide.</p> </abstract>