Association of Fusobacterium nucleatum with clinical and molecular features in colorectal serrated pathway. Issue 6 (13th March 2015)

• Record Type: Journal Article
• Authors: Ito, Miki; Kanno, Shinichi; Nosho, Katsuhiko; Sukawa, Yasutaka; Mitsuhashi, Kei; Kurihara, Hiroyoshi; Igarashi, Hisayoshi; Takahashi, Taiga; Tachibana, Mami; Takahashi, Hiroaki; Yoshii, Shinji; Takenouchi, Toshinao; Hasegawa, Tadashi; Okita, Kenji; Hirata, Koichi; Maruyama, Reo; Suzuki, Hiromu; Imai, Kohzoh; Yamamoto, Hiroyuki; Shinomura, Yasuhisa
• Is Part Of: International journal of cancer. Volume 137:Issue 6(2015:Sep. 15)
• Page Start: 1258
• Page End: 1268
• Publication Date: 2015-03-13
• Subjects: Cancer -- Periodicals; Cancer -- Prevention -- Periodicals; 616.994
• DOI: 10.1002/ijc.29488
• Languages: English
• ISSNs: 0020-7136

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Human gut microbiota is being increasingly recognized as a player in colorectal cancers (CRCs). Evidence suggests that <italic>Fusobacterium nucleatum</italic> (<italic>F. nucleatum</italic>) may contribute to disease progression and is associated with CpG island methylator phenotype (CIMP) and microsatellite instability (MSI) in CRCs; however, to date, there are no reports about the relationship between <italic>F. nucleatum</italic> and molecular features in the early stage of colorectal tumorigenesis. Therefore, we investigated the presence of <italic>F. nucleatum</italic> in premalignant colorectal lesions. In total, 465 premalignant lesions (343 serrated lesions and 122 non‐serrated adenomas) and 511 CRCs were studied. We determined the presence of <italic>F. nucleatum</italic> and analyzed its association with molecular features including CIMP, MSI and microRNA‐31 status. <italic>F. nucleatum</italic> was detected in 24% of hyperplastic polyps, 35% of sessile serrated adenomas (SSAs), 30% of traditional serrated adenomas (TSAs) and 33% of non‐serrated adenomas. <italic>F. nucleatum</italic> was more frequently detected in CIMP‐high premalignant lesions than in CIMP‐low/zero lesions (<italic>p</italic> = 0.0023). In SSAs, <italic>F. nucleatum</italic> positivity increased gradually from sigmoid colon to cecum (<italic>p</italic> = 0.042). <italic>F. nucleatum</italic> positivity was significantly higher in CRCs (56%) than in premalignant lesions of any histological type (<italic>p</italic> < 0.0001). In conclusion, <italic>F. nucleatum</italic> was identified in premalignant colorectal lesions regardless of histopathology but was more frequently associated with CIMP‐high lesions. Moreover, <italic>F. nucleatum</italic> positivity increased according to histological grade, suggesting that it may contribute to the progression of colorectal neoplasia. Our data also indicate that <italic>F. nucleatum</italic> positivity in SSAs may support the "colorectal continuum" concept.</p> </abstract>