Microbial community in persistent apical periodontitis: a 16S rRNA gene clone library analysis. (16th September 2014)

• Record Type: Journal Article
• Authors: Zakaria, M. N.; Takeshita, T.; Shibata, Y.; Maeda, H.; Wada, N.; Akamine, A.; Yamashita, Y.
• Is Part Of: International endontic journal. Volume 48:Number 8(2015:Aug.)
• Page Start: 717
• Page End: 728
• Publication Date: 2014-09-16
• Subjects: Endodontics -- Periodicals; 617.6342
• DOI: 10.1111/iej.12361
• Languages: English
• ISSNs: 0143-2885

Abstract

<abstract abstract-type="main" id="iej12361-abs-0001"> <title>Abstract</title> <sec id="iej12361-sec-0001" sec-type="section"> <title>Aim</title> <p>To characterize the microbial composition of persistent periapical lesions of root filled teeth using a molecular genetics approach.</p> </sec> <sec id="iej12361-sec-0002" sec-type="section"> <title>Methodology</title> <p>Apical lesion samples were collected from 12 patients (23–80 years old) who visited the Kyushu University Hospital for apicectomy with persistent periapical lesions associated with root filled teeth. DNA was directly extracted from each sample and the microbial composition was comprehensively analysed using clone library analysis of the 16S rRNA gene. <italic>Enterococcus faecalis</italic>, <italic> Candida albicans</italic> and specific <italic>fimA</italic> genotypes of <italic>Porphyromonas gingivalis</italic> were confirmed using polymerase chain reaction (PCR) analysis with specific primers.</p> </sec> <sec id="iej12361-sec-0003" sec-type="section"> <title>Results</title> <p>Bacteria were detected in all samples, and the dominant findings were <italic>P. gingivalis</italic> (19.9%), <italic>Fusobacterium nucleatum</italic> (11.2%) and <italic>Propionibacterium acnes</italic> (9%). Bacterial diversity was greater in symptomatic lesions than in asymptomatic ones. In addition, the following bacteria or bacterial combinations were characteristic to symptomatic lesions: <italic>Prevotella</italic> spp., <italic>Treponema</italic> spp., <italic>Peptostreptococcaceae</italic> sp. HOT‐113, <italic>Olsenella uli</italic>, <italic> Slackia exigua</italic>, <italic> Selemonas infelix</italic>, <italic> P</italic>.<italic> gingivalis</italic> with type IV <italic>fimA</italic>, and a combination of <italic>P</italic>.<italic> gingivalis</italic>, <italic> F</italic>.<italic> nucleatum</italic>, and <italic>Peptostreptococcaceae</italic> sp. HOT‐113 and predominance of <italic>Streptococcus</italic> spp. On the other hand, neither <italic>Enterococcus faecalis</italic> nor <italic>C. albicans</italic> were detected in any of the samples.</p> </sec> <sec id="iej12361-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Whilst a diverse bacterial species were observed in the persistent apical lesions, some characteristic patterns of bacterial community were found in the symptomatic lesions. The diverse variation of community indicates that bacterial combinations as a community may cause persistent inflammation in periapical tissues rather than specific bacterial species.</p> </sec> </abstract>