RNA interference of the P450 CYP6CM1 gene has different efficacy in B and Q biotypes of Bemisia tabaci. Issue 8 (8th October 2014)

• Record Type: Journal Article
• Authors: Li, Jingjing; Li, Xiaomin; Bai, Rune; Shi, Yan; Tang, Qingbo; An, Shiheng; Song, Qisheng; Yan, Fengming
• Is Part Of: Pest management science. Volume 71:Issue 8(2015:Aug.)
• Page Start: 1175
• Page End: 1181
• Publication Date: 2014-10-08
• Subjects: Pests -- Control -- Periodicals; Pesticides -- Periodicals; 632.9
• DOI: 10.1002/ps.3903
• Languages: English
• ISSNs: 1526-498X

Abstract

<abstract abstract-type="main" id="ps3903-abs-0001"> <title>Abstract</title> <sec id="ps3903-sec-0001" sec-type="section"> <title>BACKGROUND</title> <p id="ps3903-para-0001">Cytochrome P450 monooxygenases have been proven to be associated with high resistance in <italic>Bemisia tabaci</italic> B biotype (Middle East–Asia Minor 1 genetic group) and Q biotype (Mediterranean genetic group) to the neonicotinoid class of insecticides. In this study, the RNA interference (RNAi) effects on P450 <italic>CYP6CM1</italic> gene expression, mortality and pesticide‐detoxifying ability between <italic>B. tabaci</italic> B and Q biotypes were compared in an attempt to provide a basis for potential RNAi application in management of this pest.</p> </sec> <sec id="ps3903-sec-0002" sec-type="section"> <title>RESULTS</title> <p id="ps3903-para-0002">Double‐stranded RNAs (dsRNAs) of P450 <italic>CYP6CM1</italic> genes corresponding to the B and Q biotypes were synthesised using specific primers and introduced into the insect body of <italic>B. tabaci</italic> adults through membrane feeding. The results showed that dsRNAs significantly silenced the target genes in <italic>B. tabaci</italic> with dsRNA concentration or treatment time, and silencing was more effective in B biotype than in Q biotype. Feeding dsRNAs led to high mortality in both biotypes, with higher mortality in B biotype (up to 85.88%) than in Q biotype (up to 56.40%). In addition, ability to detoxify imidacloprid and nicotine was inhibited in dsRNA‐treated adults of both biotypes, more efficiently in B biotype than in Q biotype.</p> </sec> <sec id="ps3903-sec-0003" sec-type="section"> <title>CONCLUSION</title> <p id="ps3903-para-0003">RNA interference of the P450 <italic>CYP6CM1</italic> gene reduced gene expression, increased mortality, and inhibited the ability to detoxify a pesticide or a plant secondary metabolite in both biotypes of <italic>B. tabaci</italic>, with better efficacy in B biotype than in Q biotype. © 2014 Society of Chemical Industry</p> </sec> </abstract>