Overexpression of patatin‐related phospholipase AIIIδ altered plant growth and increased seed oil content in camelina. Issue 6 (29th December 2014)

• Record Type: Journal Article
• Authors: Li, Maoyin; Wei, Fang; Tawfall, Amanda; Tang, Michelle; Saettele, Allison; Wang, Xuemin
• Is Part Of: Plant biotechnology journal. Volume 13:Issue 6(2015:Aug.)
• Page Start: 766
• Page End: 778
• Publication Date: 2014-12-29
• Subjects: Plant biotechnology -- Periodicals; Plant genetic engineering -- Periodicals; 630.272
• DOI: 10.1111/pbi.12304
• Languages: English
• ISSNs: 1467-7644

Abstract

<abstract abstract-type="main" id="pbi12304-abs-0001"> <title>Summary</title> <p> <italic>Camelina sativa</italic> is a Brassicaceae oilseed species being explored as a biofuel and industrial oil crop. A growing number of studies have indicated that the turnover of phosphatidylcholine plays an important role in the synthesis and modification of triacylglycerols. This study manipulated the expression of a patatin‐related phospholipase AIIIδ (<italic>pPLAIII</italic>δ) in camelina to determine its effect on seed oil content and plant growth. Constitutive overexpression of <italic>pPLAIII</italic>δ under the control of the constitutive cauliflower mosaic 35S promoter resulted in a significant increase in seed oil content and a decrease in cellulose content. In addition, the content of major membrane phospholipids, phosphatidylcholine and phosphatidylethanolamine, in <italic>35S::pPLAIII</italic>δ plants was increased. However, these changes in <italic>35S::pPLAIII</italic>δ camelina were associated with shorter cell length, leaves, stems, and seed pods and a decrease in overall seed production. When <italic>pPLAIII</italic>δ was expressed under the control of the seed specific, β‐conglycinin promoter, the seed oil content was increased without compromising plant growth. The results suggest that <italic>pPLAIII</italic>δ alters the carbon partitioning by decreasing cellulose content and increasing oil content in camelina.</p> </abstract>