Loss expression of micro ribonucleic acid (miRNA)‐200c induces adverse post‐surgical prognosis of advanced stage non‐small cell lung carcinoma and its potential relationship with ETAR messenger RNA. Issue 4 (July 2015)

• Record Type: Journal Article
• Authors: Zhao, Jinpeng; Zhao, Yandong; Wang, Zizong; Xuan, Yunpeng; Luo, Yiren; Jiao, Wenjie
• Is Part Of: Thoracic cancer. Volume 6:Issue 4(2015)
• Page Start: 421
• Page End: 426
• Publication Date: 2015-07
• Subjects: Chest -- Cancer -- Periodicals; Chest -- Cancer -- Treatment -- Periodicals; Chest -- Surgery -- Periodicals; 616.99494005
• DOI: 10.1111/1759-7714.12193
• Languages: English
• ISSNs: 1759-7706

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="tca12193-sec-0001" sec-type="section"> <title>Background</title> <p>Non‐small cell lung cancer (NSCLC) is the leading cause of cancer mortality worldwide. As micro ribonucleic acid (miRNA)‐200 and ET<sub>A</sub>R may play an essential role in the process of epithelial to mesenchymal transition (EMT) simultaneously, the purpose of this study was to detect the expression of miRNA‐200c and ET<sub>A</sub>R messenger (m)RNA and assess their prognostic significance in early stage NSCLC.</p> </sec> <sec id="tca12193-sec-0002" sec-type="section"> <title>Methods</title> <p>Our study included 78 advanced stage (II<sub>B</sub>, III<sub>A</sub>, III<sub>B</sub>) NSCLC patients. All patients were smokers. Using quantitative reverse transcriptase polymerase chain reaction analysis, we detected the expression of miRNA‐200c and ET<sub>A</sub>R mRNA and assessed their correlation by χ<sup>2</sup> test. Time to progression was used as the recurrent index and was assessed by univariate and multivariate analysis in the Cox hazard model.</p> </sec> <sec id="tca12193-sec-0003" sec-type="section"> <title>Results</title> <p>Both miRNA‐200c and ET<sub>A</sub>R mRNA expression are associated with N stage and tumor node metastasis (TNM) stage in a series of advanced NSCLC patients. Among N stage and TNM stage patients, significant differences were found in II<sub>B</sub> (<italic>P</italic> = 0.0126), III<sub>B</sub> (<italic>P</italic> = 0.0107) and N<sub>0</sub> (<italic>P</italic> = 0.0023) and in N<sub>1</sub> + N<sub>2</sub> groups (<italic>P</italic> = 0.0133). Using both univariate and multivariate survival analyses, we found that miRNA‐200c (hazard ratio [HR] = 0.352, 95% confidence interval [CI]: 0.187–0.662) and ET<sub>A</sub>R mRNA (HR = 2.500 95% CI: 1.345–4.647) were independent prognostic factors, independent of TNM stage (HR = 2.414, 95% CI: 1.600–3.642) and differentiation (HR = 1.530, 95% CI: 1.050–2230).</p> </sec> <sec id="tca12193-sec-0004" sec-type="section"> <title>Conclusions</title> <p> miRNA‐200c induces an expedient surgical survival, whereas ET<sub>A</sub>R mRNA has the reverse prognosis in advanced stage NSCLC patients. A potential relationship exists in that miRNA‐200c targets ET<sub>A</sub>R mRNA during EMT.</p> </sec> </abstract>