Potential anti‐cancer effect of curcumin in human lung squamous cell carcinoma. Issue 4 (26th January 2015)

• Record Type: Journal Article
• Authors: Zhao, Wei; Wang, Yan; Wang, Ying; Gao, Nan; Han, Zhifeng; Yu, Haixiang
• Is Part Of: Thoracic cancer. Volume 6:Issue 4(2015)
• Page Start: 508
• Page End: 516
• Publication Date: 2015-01-26
• Subjects: Chest -- Cancer -- Periodicals; Chest -- Cancer -- Treatment -- Periodicals; Chest -- Surgery -- Periodicals; 616.99494005
• DOI: 10.1111/1759-7714.12222
• Languages: English
• ISSNs: 1759-7706

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="tca12222-sec-0001" sec-type="section"> <title>Background</title> <p>To explore the molecular mechanisms of the anti‐cancer effect of curcumin in human lung squamous cell carcinoma (LSQCC) SK‐MES‐1 cells.</p> </sec> <sec id="tca12222-sec-0002" sec-type="section"> <title>Methods</title> <p>Cell viability was determined using MTT assay. Ribonucleic acid sequencing was performed to measure expression levels of transcripts in LSQCC cells treated with 15 μmol/L curcumin (treatment groups) or an equal amount of dimethylsulfoxide (control). Cuffdiff software was used to identify differentially expressed genes (DEGs) in treatment groups, followed by enrichment analysis of DEGs using the Database for Annotation, Visualization and Integration Discovery. The protein‐protein interaction (PPI) networks for up and downregulated DEGs were constructed by Cytoscape software using Search Tool for the Retrieval of Interacting Genes data to identify hub nodes.</p> </sec> <sec id="tca12222-sec-0003" sec-type="section"> <title>Results</title> <p>Curcumin significantly reduced cell viability in LSQCC cells. In total, 380 DEGs including 154 upregulated and 126 downregulated genes were found in the treatment groups. The upregulated genes were enriched in base excision repair (BER, such as <italic>PCNA</italic>, <italic>POLL</italic>, and <italic>MUTYH</italic>) and Janus kinase‐signal transducer and activator of transcription (JAT‐STAT) signaling pathways (such as <italic>AKT1</italic> and <italic>STAT5A</italic>), while the downregulated genes were enriched in nine pathways, including the vascular endothelial growth factor (VEGF) signaling pathway (such as <italic>PTK2</italic>, <italic>VEGFA</italic>, <italic>MAPK1</italic>, and <italic>MAPK14</italic>) and mitogen‐activated protein kinase (MAPK) signaling pathway (<italic>ARRB2</italic>, <italic>MAPK1, MAPK14</italic>, <italic>and NFKB1</italic>). <italic>PCNA</italic> and <italic>AKT1</italic> were the hub nodes in the PPI network of upregulated genes while <italic>MAPK1</italic>, <italic>MAPK14</italic>, <italic>VEGFA</italic>, and <italic>NFKB1</italic> were the hub nodes in the PPI network of downregulated genes.</p> </sec> <sec id="tca12222-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Curcumin might exert anti‐cancer effects on LSQCC via regulating BER, JAT‐STAT, VEGF, and MAPK signaling pathways.</p> </sec> </abstract>