Hydrogen sulfide regulates abiotic stress tolerance and biotic stress resistance in Arabidopsis. Issue 7 (13th January 2015)

• Record Type: Journal Article
• Authors: Shi, Haitao; Ye, Tiantian; Han, Ning; Bian, Hongwu; Liu, Xiaodong; Chan, Zhulong
• Is Part Of: Journal of integrative plant biology. Volume 57:Issue 7(2015)
• Page Start: 628
• Page End: 640
• Publication Date: 2015-01-13
• Subjects: Plants -- Periodicals; Plants -- China -- Periodicals; Electronic journals; 580.5
• DOI: 10.1111/jipb.12302
• Languages: English
• ISSNs: 1672-9072

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="jipb12302-sec-0001" sec-type="section"> <p>Hydrogen sulfide (H<sub>2</sub>S) is an important gaseous molecule in various plant developmental processes and plant stress responses. In this study, the transgenic <italic>Arabidopsis thaliana</italic> plants with modulated expressions of two cysteine desulfhydrases, and exogenous H<sub>2</sub>S donor (sodium hydrosulfide, NaHS) and H<sub>2</sub>S scavenger (hypotaurine, HT) pre‐treated plants were used to dissect the involvement of H<sub>2</sub>S in plant stress responses. The cysteine desulfhydrases overexpressing plants and NaHS pre‐treated plants exhibited higher endogenous H<sub>2</sub>S level and improved abiotic stress tolerance and biotic stress resistance, while cysteine desulfhydrases knockdown plants and HT pre‐treated plants displayed lower endogenous H<sub>2</sub>S level and decreased stress resistance. Moreover, H<sub>2</sub>S upregulated the transcripts of multiple abiotic and biotic stress‐related genes, and inhibited reactive oxygen species (ROS) accumulation. Interestingly, MIR393‐mediated auxin signaling including <italic>MIR393a</italic>/<italic>b</italic> and their target genes (<italic>TIR1</italic>, <italic>AFB1</italic>, <italic>AFB2</italic>, and <italic>AFB3</italic>) was transcriptionally regulated by H<sub>2</sub>S, and was related with H<sub>2</sub>S‐induced antibacterial resistance. Moreover, H<sub>2</sub>S regulated 50 carbon metabolites including amino acids, organic acids, sugars, sugar alcohols, and aromatic amines. Taken together, these results indicated that cysteine desulfhydrase and H<sub>2</sub>S conferred abiotic stress tolerance and biotic stress resistance, via affecting the stress‐related gene expressions, ROS metabolism, metabolic homeostasis, and MIR393‐targeted auxin receptors.</p> </sec> </abstract>