TRIB3 mediates the expression of Wnt5a and activation of nuclear factor‐κB in Porphyromonas endodontalis lipopolysaccharide‐treated osteoblasts. (9th March 2015)

• Record Type: Journal Article
• Authors: Yu, Y.; Qiu, L.; Guo, J.; Yang, D.; Qu, L.; Yu, J.; Zhan, F.; Xue, M.; Zhong, M.
• Is Part Of: Molecular oral microbiology. Volume 30:Number 4(2015)
• Page Start: 295
• Page End: 306
• Publication Date: 2015-03-09
• Subjects: Mouth -- Microbiology -- Periodicals; Respiratory infections -- Microbiology -- Periodicals; Mouth -- Diseases -- Immunological aspects -- Periodicals; 617.522
• DOI: 10.1111/omi.12094
• Languages: English
• ISSNs: 2041-1006

Abstract

<abstract abstract-type="main" id="omi12094-abs-0001"> <title>Summary</title> <p> <italic>Porphyromonas endodontalis</italic> lipopolysaccharide (LPS) is considered to be correlated with the progression of bone resorption in periodontal and periapical diseases. Wnt5a has recently been implicated in inflammatory processes, but its role is unclear as a <italic>P. endodontalis</italic>LPS‐induced mediator in osteoblasts. Tribbles homolog 3 (TRIB3) encodes a pseudokinase and has been linked to inflammation in certain situations. Here, we found that <italic>P. endodontalis</italic>LPS induced Wnt5a expression in a dose‐ and time‐dependent manner and it also upregulated translocation, phosphorylation and transcriptional activity of nuclear factor‐κB (NF‐κB) in MC3T3‐E1 cells. Bay 11‐7082 blocked the translocation of NF‐κB and Wnt5a expression induced by <italic>P. endodontalis</italic>LPS. Chromatin immunoprecipitation assay further established that induction of Wnt5a by <italic>P. endodontalis</italic>LPS was mediated through the NF‐κB p65 subunit. Additionally, <italic>P. endodontalis</italic>LPS increased expression of TRIB3 in osteoblasts after 10 h simulated time. Overexpression of TRIB3 enhanced NF‐κB phosphorylation and Wnt5a induction, whereas knockdown of TRIB3 inhibited NF‐κB phosphorylation and Wnt5a expression in <italic>P. endodontalis</italic>LPS‐stimulated osteoblasts. These results suggest that <italic>P. endodontalis</italic>LPS has the ability to promote the expression of Wnt5a in mouse osteoblasts, and this induction is mainly mediated by NF‐κB pathway. TRIB3 seems to modulate the sustained expression of Wnt5a in osteoblasts stimulated by <italic>P. endodontalis</italic>LPS, as well as regulating NF‐κB phosphorylation.</p> </abstract>