Altered cytokine profiles in children with indeterminate quantiferon results and common infections. Issue 2 (August 2015)

• Record Type: Journal Article
• Authors: Jeljeli, Mohamed; Guérin-El Khourouj, Valérie; de Lauzanne, Agathe; Armand, Marine; Chhor, Vibol; Pédron, Béatrice; Dauger, Stéphane; Viala, Jérôme; Gressens, Pierre; Faye, Albert; Sterkers, Ghislaine
• Is Part Of: Journal of infection. Volume 71:Issue 2(2015)
• Page Start: 250
• Page End: 257
• Publication Date: 2015-08
• Subjects: Infection -- Periodicals; Bacterial Infections -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.905
• DOI: 10.1016/j.jinf.2015.03.014
• Languages: English
• ISSNs: 0163-4453

Abstract

<abstract xml:lang="en" abstract-type="author" id="abs0010"> <title id="sectitle0010">Summary</title> <sec> <title id="sectitle0015">Objectives</title> <p id="abspara0010">An increased rate of indeterminate quantiferon results (low IFN-γ release in the phytohemagglutinin-stimulated tube) has been reported in children with clinical signs compatible with tuberculosis but with the final diagnosis of infectious diseases different from tuberculosis. Here, we addressed the mechanisms involved and assessed potential alternative biomarkers to overcome indeterminate quantiferon results under these conditions.</p> </sec> <sec> <title id="sectitle0020">Methods</title> <p id="abspara0015">Cytokine concentrations were measured in residual plasma from quantiferon assays performed in immunocompetent children (cases, median age: 3 years 9 months) with indeterminate results and community acquired pneumonia (n = 7) or meningoencephalitis (n = 1). Controls were age-matched immunocompetent children with determinate quantiferon results (infected with <italic>mycobacterium tuberculosis</italic>, n = 7 or not, n = 8).</p> </sec> <sec> <title id="sectitle0025">Results</title> <p id="abspara0020">Lower IFN-γ expression in phytohemagglutinin-stimulated cultures from cases was accompanied by lower Th1 (IL-2, TNF-α, IP-10) and Th2 (IL-5, IL-13), but similar IL-10 secretion capacities as the controls.</p> </sec> <sec> <title id="sectitle0030">Conclusions</title> <p id="abspara0025">A state of hyporesponsiveness that resembles the concept of immunoparalysis in severe infection was observed in children with milder infections. Though IP-10, IL-2, IL-5 and IL-13 were confirmed as promising alternative biomarkers for discriminating controls with and without tuberculosis in this study, defective induction of these biomarkers by phytohemagglutinin in cases precluded their usefulness in overcoming quantiferon indeterminate results in the above-mentioned clinical conditions.</p> </sec> </abstract>