ISAC's Gating‐ML 2.0 data exchange standard for gating description. (14th May 2015)

• Record Type: Journal Article
• Authors: Spidlen, Josef; Moore, Wayne; ISAC Data Standards Task Force; Brinkman, Ryan R.; Brinkman, Ryan R.; Aghaeepour, Nima; Finak, Greg; Gottardo, Raphael; Mosmann, Tim; Scheuermann, Richard H.
• Is Part Of: Cytometry. Volume 87:Number 7(2015)
• Page Start: 683
• Page End: 687
• Publication Date: 2015-05-14
• Subjects: Flow cytometry -- Periodicals; Imaging systems in biology -- Periodicals; Imaging systems in medicine -- Periodicals; Diagnostic imaging -- Periodicals; 571.605
• DOI: 10.1002/cyto.a.22690
• Languages: English
• ISSNs: 1552-4922

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>The lack of software interoperability with respect to gating has traditionally been a bottleneck preventing the use of multiple analytical tools and reproducibility of flow cytometry data analysis by independent parties. To address this issue, ISAC developed Gating‐ML, a computer file format to encode and interchange gates. Gating‐ML 1.5 was adopted and published as an ISAC Candidate Recommendation in 2008. Feedback during the probationary period from implementors, including major commercial software companies, instrument vendors, and the wider community, has led to a streamlined Gating‐ML 2.0. Gating‐ML has been significantly simplified and therefore easier to support by software tools. To aid developers, free, open source reference implementations, compliance tests, and detailed examples are provided to stimulate further commercial adoption. ISAC has approved Gating‐ML as a standard ready for deployment in the public domain and encourages its support within the community as it is at a mature stage of development having undergone extensive review and testing, under both theoretical and practical conditions. © 2015 International Society for Advancement of Cytometry</p> </abstract>