Genotype of FLOWERING LOCUS T homologue contributes to flowering time differences in wild and cultivated roses. (19th January 2015)

• Record Type: Journal Article
• Authors: Otagaki, S.; Ogawa, Y.; Hibrand‐Saint Oyant, L.; Foucher, F.; Kawamura, K.; Horibe, T.; Matsumoto, S.; Peeters, T.
• Is Part Of: Plant biology. Volume 17:Number 4(2015:Jul.)
• Page Start: 808
• Page End: 815
• Publication Date: 2015-01-19
• Subjects: Botany -- Periodicals; Plants -- genetics -- Periodicals; Plants -- growth & development -- Periodicals; Plant Proteins -- Periodicals; Gene Expression Regulation, Plant -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1111/plb.12299
• Languages: English
• ISSNs: 1435-8603

Abstract

<abstract abstract-type="main" id="plb12299-abs-0001"> <title>Abstract</title> <p>Rose flowers have long delighted humans as ornamental plants. To improve the ornamental value of roses it is necessary to understand the regulatory mechanisms of flowering. We previously found that flowering time is controlled by three minor quantitative trait loci (QTLs) and a major QTL co‐localised with <italic>RoFT</italic>. In this study, we isolated three <italic>RoFT</italic> alleles encoding completely identical amino acid sequences from the parents of a mapping population. Correlation analysis of the <italic>RoFT</italic> genotypes and flowering time phenotypes in the mapping population showed that the <italic>RoFT_f</italic> and <italic>RoFT_g</italic> alleles contribute to the early‐flowering phenotype, while the <italic>RoFT_e</italic> allele contributes to the late‐flowering phenotype. We developed two novel cleaved amplified polymorphic sequence (CAPS) markers based on the genomic sequences of the <italic>RoFT</italic> alleles and clearly showed that the relationship between <italic>RoFT</italic> genotype and flowering time was applicable to 12 of 13 cultivated roses grown at the Higashiyama Botanical Gardens, Japan. Allele‐specific expression analysis using a reverse transcription CAPS assay suggested that these <italic>RoFT</italic> alleles are regulated differentially at the transcription level. Furthermore, transgenic <italic>Arabidopsis thaliana</italic> plants ectopically expressing the <italic>RoFT</italic> gene showed an early‐flowering phenotype. Conversely, in roses, <italic>RoFT</italic> was continuously expressed after floral bud formation, and <italic>RoFT</italic> transcript accumulation reached its peak after that of the floral meristem identity gene <italic>RoAP1b</italic>. These data suggest that RoFT may be essential not only for floral transition but also for normal floral development and flowering in roses.</p> </abstract>