Development of a general‐purpose method for cell purification using Cre/loxP‐mediated recombination. Issue 6 (11th June 2015)

• Record Type: Journal Article
• Authors: Kuroki, Shunsuke; Akiyoshi, Mika; Ideguchi, Ko; Kitano, Satsuki; Miyachi, Hitoshi; Hirose, Michiko; Mise, Nathan; Abe, Kuniya; Ogura, Atsuo; Tachibana, Makoto
• Is Part Of: Genesis. Volume 53:Issue 6(2015:Jun.)
• Page Start: 387
• Page End: 393
• Publication Date: 2015-06-11
• Subjects: Developmental genetics -- Periodicals; Genetics -- Periodicals; Developmental biology -- Periodicals; Embryology -- Periodicals; Genetic regulation -- Periodicals; 576.5
• DOI: 10.1002/dvg.22863
• Languages: English
• ISSNs: 1526-954X

Abstract

<abstract abstract-type="main"> <title>Summary</title> <p>A mammalian body is composed of more than 200 different types of cells. The purification of a certain cell type from tissues/organs enables a wide variety of studies. One popular cell purification method is immunological isolation, using antibodies against specific cell surface antigens. However, this is not a general‐purpose method, since suitable antigens have not been found in certain cell types, including embryonic gonadal somatic cells and Sertoli cells. To address this issue, we established a knock‐in mouse line, named <italic>R26 KI</italic>, designed to express the human cell surface antigen hCD271 through Cre/loxP‐mediated recombination. First, we used the <italic>R26 Kl</italic> mouse line to purify embryonic gonadal somatic cells. Gonadal somatic cells were purified from the <italic>R26 KI</italic>; <italic>Nr5a1‐Cre</italic>‐transgenic (tg) embryos almost equally as efficiently as from <italic>Nr5a1‐hCD271</italic>‐tg embryos. Second, we used the <italic>R26 KI</italic> mouse line to purify Sertoli cells successfully from <italic>R26 KI</italic>; <italic>Amh‐Cre</italic>‐tg testes. In summary, we propose that the <italic>R26 KI</italic> mouse line is a powerful tool for the purification of various cell types. genesis 53:387–393, 2015. © 2015 Wiley Periodicals, Inc.</p> </abstract>