Assessment of ex vivo perfused liver health by Raman spectroscopy. (28th April 2015)

• Record Type: Journal Article
• Authors: Zu, Theresah N. K.; Athamneh, Ahmad I. M.; Collakova, Eva; Robertson, John; Hawken, Thomas; Aardema, Charles; Senger, Ryan S.
• Is Part Of: Journal of Raman spectroscopy. Volume 46:Number 6(2015:Jun.)
• Page Start: 551
• Page End: 558
• Publication Date: 2015-04-28
• Subjects: Raman spectroscopy -- Periodicals; 535.846
• DOI: 10.1002/jrs.4688
• Languages: English
• ISSNs: 0377-0486

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Raman spectroscopy was applied in this research to monitor the overall health and degradation of porcine livers perfused <italic>ex vivo</italic> using the VasoWaveW® perfusion system. A novel Raman‐based diagnostic analysis was developed that enables near real‐time and label‐free monitoring of organ health during <italic>ex vivo</italic> perfusion designed to extend the useful life of the organ for transplantation. Multivariate statistical analysis of Raman spectra of organ perfusate fluid, using a combination of principal component analysis and linear discriminant analysis, proved to be an effective technique to assess the degradation properties of the livers. Three livers (with replicates) were perfused <italic>ex vivo</italic> under different pressures and temperatures and were compared with a 24‐h time‐course. Results indicated that perfusion pressure was a more significant factor in organ degradation than was temperature. In addition, a non‐linear degradation profile was identified for all three perfused livers, and this profile was different for individual livers, demonstrating the time‐dependent transition from its initial 'healthy' state towards a more 'unhealthy' degenerative state at 24 h. The Raman spectroscopy‐based approach described here has potential applications in perfusion and diagnostic instrumentation that can be used in near real‐time during organ transit and in operating rooms to help identify appropriately healthy organs for transplantation. Copyright © 2015 John Wiley & Sons, Ltd.</p> </abstract>