Extraction of antibiotic zwittermicin A from Bacillus thuringiensis by macroporous resin and silica gel column chromatography. (5th September 2014)

• Record Type: Journal Article
• Authors: Hao, Zaibin; Yan, Li; Liu, Jianguo; Song, Fuping; Zhang, Jie; Li, Xia
• Is Part Of: Biotechnology and applied biochemistry. Volume 62:Number 3(2015)
• Page Start: 369
• Page End: 374
• Publication Date: 2014-09-05
• Subjects: Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Genetic Techniques -- Periodicals; Microbiological Techniques -- Periodicals; 660.6
• DOI: 10.1002/bab.1277
• Languages: English
• ISSNs: 0885-4513

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>To establish a production process capable of providing refined zwittermicin A (ZwA) on a large scale, the macroporous resin and silica gel column chromatography were used to separate and purify the antibiotic ZwA from the fermentation broth of <italic>Bacillus thuringiensis</italic> HD‐1. The result of high‐performance liquid chromatography–mass spectrometry after purification suggests that the samples of ZwA were of high purity, 89%, and the average yield was 20 mg L⁻¹. <italic>Erwinia herbicola</italic> LS005, <italic>Escherichia coli</italic>, <italic>Staphylococcus aureus</italic>, and <italic>Bacillus subtilis</italic> were used to assess the toxicity of ZwA. The antibiotic had strong antibacterial activity against <italic>E. herbicola</italic> LS005 and a color reaction with ninhydrin.</p> </abstract>