Identification of a functional antioxidant responsive element in the promoter of the Chinese hamster carbonyl reductase 3 (Chcr3) gene. (23rd February 2015)

• Record Type: Journal Article
• Authors: Miura, Takeshi; Taketomi, Ayako; Nakabayashi, Toshikatsu; Nishinaka, Toru; Terada, Tomoyuki
• Is Part Of: Cell biology international. Volume 39:Number 7(2015)
• Page Start: 808
• Page End: 815
• Publication Date: 2015-02-23
• Subjects: Cytology -- Periodicals; Cells -- Periodicals; 571.605
• DOI: 10.1002/cbin.10450
• Languages: English
• ISSNs: 1065-6995

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="cbin10450-sec-0001" sec-type="section"> <p>CHCR3, a member of the short‐chain dehydrogenase/reductase superfamily, is a carbonyl reductase 3 enzyme in Chinese hamsters. Carbonyl reductase 3 in humans has been believed to involve the metabolism and/or pharmacokinetics of anthracycline drugs, and the mechanism underlying the gene regulation has been investigated. In this study, the nucleotide sequence of the <italic>Chcr3</italic> promoter was originally determined, and its promoter activity was characterised. The proximal promoter region is TATA‐less and GC‐rich, similar to the promoter region of human carbonyl reductase 3. Cobalt stimulated the transcriptional activity of the <italic>Chcr3</italic> gene. The results of a luciferase gene reporter assay demonstrated that cobalt‐induced stimulation required an antioxidant responsive element. Forced expression of Nrf2, the transcription factor that binds to antioxidant responsive elements, enhanced the transcriptional activity of the <italic>Chcr3</italic> gene. These results suggest that cobalt induces the expression of the <italic>Chcr3</italic> gene via the Nrf2‐antioxidant responsive element pathway.</p> </sec> </abstract>