Identification of LncRNAs/mRNAs related to endometrium function regulated by Homeobox A10 in Ishikawa cells. (13th April 2015)

• Record Type: Journal Article
• Authors: Lin, Ruiyi; Zhao, Changzhi; Jing, Lu; Zhao, Shuhong
• Is Part Of: Cell biology international. Volume 39:Number 7(2015)
• Page Start: 842
• Page End: 851
• Publication Date: 2015-04-13
• Subjects: Cytology -- Periodicals; Cells -- Periodicals; 571.605
• DOI: 10.1002/cbin.10458
• Languages: English
• ISSNs: 1065-6995

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="cbin10458-sec-0001" sec-type="section"> <p>As a well‐known transcription factor, Homeobox A10 (<italic>HOXA10</italic>) regulates a large number of downstream target genes, leading to the proper function development of endometrium for embryo implantation. The change of <italic>HOXA10</italic> gene expression level can alter the expressions of many other genes, including coding and noncoding transcripts. In our study, mRNA and LncRNA expression profiles screening was performed by microarray when the <italic>HOXA10</italic> gene expression level increased in Ishikawa cells. A total of 907 mRNAs and 1, 026 LncRNAs were identified as differentially expressed transcripts (Fold Change ≥2, <italic>P‐</italic>value &lt;0.05, and Q‐value &lt;0.05) between <italic>HOXA10</italic> overexpressed and control Ishikawa cells. Further analysis identified that these mRNAs participated in various biological processes, such as blood vessel development, cell adhesion, cell cycle, etc. Also, 14 enhancer‐like LncRNAs and 108 LincRNAs with their nearby mRNAs were identified as coregulated transcripts. Our results showed that the mRNA and LncRNA expression profiles differed significantly between the two groups and provided useful information for further studying the molecular mechanisms of <italic>HOXA10</italic> in endometrium.</p> </sec> </abstract>