An in vitro model of mesenchymal stem cell targeting using magnetic particle labelling. (27th December 2012)

• Record Type: Journal Article
• Authors: El Haj, Alicia J.; Glossop, John R.; Sura, Harpal S.; Lees, Martin R.; Hu, Bin; Wolbank, Susanne; van Griensven, Martijn; Redl, Heinz; Dobson, Jon
• Is Part Of: Journal of tissue engineering and regenerative medicine. Volume 9:Number 6(2015:Jun.)
• Page Start: 724
• Page End: 733
• Publication Date: 2012-12-27
• Subjects: Tissue engineering -- Periodicals; Regeneration (Biology) -- Periodicals; 610.28
• DOI: 10.1002/term.1636
• Languages: English
• ISSNs: 1932-6254

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>The specific targeting of cells to sites of tissue damage <italic>in vivo</italic> is a major challenge precluding the success of stem cell‐based therapies. Magnetic particle‐based targeting may provide a solution. Our aim was to provide a model system to study the trapping and potential targeting of human mesenchymal stem cells (MSCs) during <italic>in vitro</italic> fluid flow, which ultimately will inform cell targeting <italic>in vivo</italic>. In this system magnet arrays were used to trap superparamagnetic iron oxide particle‐doped MSCs. The <italic>in vitro</italic> experiments demonstrated successful cell trapping, where the volume of cells trapped increased with magnetic particle concentration and decreased with increasing flow rate. Analysis of gene expression revealed significant increases in <italic>COL1A2</italic> and <italic>SOX9</italic>. Using principles established <italic>in vitro</italic>, a proof‐of‐concept <italic>in vivo</italic> experiment demonstrated that magnetic particle‐doped, luciferase‐expressing MSCs were trapped by an implanted magnet in a subcutaneous wound model in nude mice. Our results demonstrate the effectiveness of using an <italic>in vitro</italic> model for testing superparamagnetic iron oxide particles to develop successful MSC targeting strategies during fluid flow, which ultimately can be translated to <italic>in vivo</italic> targeted delivery of cells via the circulation in a variety of tissue‐repair models. Copyright © 2012 John Wiley & Sons, Ltd.</p> </abstract>