Neutrophils exacerbate tuberculosis infection in genetically susceptible mice. Issue 4 (July 2015)

• Record Type: Journal Article
• Authors: Yeremeev, Vladimir; Linge, Irina; Kondratieva, Tatiana; Apt, Alexander
• Is Part Of: Tuberculosis. Volume 95:Issue 4(2015)
• Page Start: 447
• Page End: 451
• Publication Date: 2015-07
• Subjects: 616.995
• DOI: 10.1016/j.tube.2015.03.007
• Languages: English
• ISSNs: 1472-9792

Abstract

<abstract xml:lang="en" abstract-type="author" id="abs0010"> <title id="sectitle0010">Summary</title> <sec> <p id="abspara0010">Mice of the I/St inbred strain genetically hyper-susceptible to TB infection and prone to form neutrophil-abundant necrotic lung lesions and relatively resistant mice of the C57BL/6 (B6) strain were infected with 100 CFU of <italic>M. tuberculosis</italic> H37Rv. To verify the role of neutrophils in TB immunity, we selectively depleted neutrophils from infected mice with highly specific 1A8 anti-Ly6G antibodies at day 2 and 6 post-challenge. Depletion of neutrophils resulted in reduced lung tissue pathology, mycobacterial CFU counts and an increase of the survival time in genetically susceptible I/St, but not in B6 mice. Furthermore, we demonstrated that <italic>in vivo</italic> neutrophil depletion at the onset of TB infection results in a significant increase in numbers of mycobacteria-specific IFN-γ-producing T-cells at the time point when the acquired immunity to mycobacteria is fully developed. These results suggest antagonistic activity of neutrophils and immune T-cells in the course of TB infection and provide further evidence of deleterious rather than protective role of the former.</p> </sec> </abstract>