The fibroblast growth factor-2 arrests Mycobacterium avium sp. paratuberculosis growth and immunomodulates host response in macrophages. Issue 4 (July 2015)

• Record Type: Journal Article
• Authors: Wang, Jianjun; Wang, Zeyou; Yao, Yongliang; Wu, Jianhong; Tang, Xin; Gu, Tao; Li, Guangxin
• Is Part Of: Tuberculosis. Volume 95:Issue 4(2015)
• Page Start: 505
• Page End: 514
• Publication Date: 2015-07
• Subjects: 616.995
• DOI: 10.1016/j.tube.2015.04.006
• Languages: English
• ISSNs: 1472-9792

Abstract

<abstract xml:lang="en" abstract-type="author" id="abs0010"> <title id="sectitle0010">Summary</title> <sec> <p id="abspara0010"> <italic>Mycobacterium tuberculosisis</italic> (<italic>M. tb</italic>) epidemic is one of the most severe health problem worldwide, while mechanisms underlying its pathogenesis and host immune responses remain unclear. <italic>Mycobacterium avium</italic> (<italic>M. avium</italic>), a mycobacterial species related to <italic>M. tb</italic>, shares similarities with <italic>M. tb</italic> in many ways. In this study, using <italic>M. avium</italic> infection of macrophages as a model, we systematically studied the effect of fibroblast growth factor-2 (FGF-2) on <italic>M. avium</italic> infection of macrophages. Our results showed that <italic>M. avium</italic> infection could increase FGF-2 expression on both mRNA and protein levels. <italic>M. avium</italic> infection elevated TNF-α and IFN-γ production while the addition of FGF-2 could further increase TNF-α but not IFN-γ level. <italic>M. avium</italic> infection could increase the expression of oxygen/nitrogen metabolism proteins iNOS and SOD-1, and FGF-2 had additive effect on the expression of these two proteins. <italic>M. avium</italic> infection had inhibitive effect on actin expression while FGF-2 could partly counteract such inhibition. Moreover, FGF-2 could inhibit <italic>M. avium</italic> proliferation in macrophages. Our results together indicate that macrophage-secreted FGF-2 upon <italic>M. avium</italic> infection could suppress <italic>M. avium</italic> proliferation through various ways including cytokine production, enhancement of phagocytosis as well as oxygen/nitrogen metabolism.</p> </sec> </abstract>