Adenosine A2a receptor stimulation prevents proteinuria in diabetic rats by promoting an anti‐inflammatory phenotype without affecting oxidative stress. (6th May 2015)

• Record Type: Journal Article
• Authors: Persson, P.; Friederich‐Persson, M.; Fasching, A.; Hansell, P.; Inagi, R.; Palm, F.
• Is Part Of: Acta physiologica. Volume 214:Number 3(2015:Jul.)
• Page Start: 311
• Page End: 318
• Publication Date: 2015-05-06
• Subjects: Physiology -- Periodicals; Physiology -- Research -- Periodicals; 612
• DOI: 10.1111/apha.12511
• Languages: English
• ISSNs: 1748-1708

Abstract

<abstract abstract-type="main" id="apha12511-abs-0001"> <title>Abstract</title> <sec id="apha12511-sec-0001" sec-type="section"> <title>Aim</title> <p>Diabetic patients are at increased risk for kidney disease. There is presently no clinical treatment available that effectively protects kidney function in diabetics. This study investigates whether chronic stimulation of the adenosine A<sub>2a</sub> receptor (A<sub>2a</sub>AR) protects kidney function in insulinopenic diabetic rats.</p> </sec> <sec id="apha12511-sec-0002" sec-type="section"> <title>Methods</title> <p>Streptozotocin‐induced diabetic rats and corresponding controls were chronically treated with the adenosine A<sub>2a</sub>AR agonist CGS21680 throughout the four‐week diabetes duration. Kidney function was thereafter investigated, and urine and plasma samples were collected for analysis of protein, oxidative stress and inflammatory markers.</p> </sec> <sec id="apha12511-sec-0003" sec-type="section"> <title>Results</title> <p>Glomerular filtration rate, renal blood flow, filtration fraction and diabetes‐induced kidney hypoxia were all unaffected by chronic A<sub>2a</sub>AR stimulation. Furthermore, diabetic rats had increased oxidative stress, which was further increased by chronic A<sub>2a</sub>AR stimulation. However, the 10‐fold increased urinary protein excretion observed in the diabetic rats was completely prevented by chronic A<sub>2a</sub>AR stimulation. These beneficial effects were accompanied by reduced levels of the pro‐inflammatory TNF‐<italic>α</italic> and increased levels of the anti‐inflammatory IL‐10 as well as decreased infiltration of macrophages, glomerular damage and basement membrane thickness.</p> </sec> <sec id="apha12511-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Chronic A<sub>2a</sub>AR stimulation prevents proteinuria and glomerular damage in experimental diabetes via an anti‐inflammatory mechanism independent of oxidative stress and kidney hypoxia.</p> </sec> </abstract>