Estrogen and progesterone receptor subtype expression in granulosa cells from women with polycystic ovary syndrome. (May 2015)

• Record Type: Journal Article
• Authors: Artimani, Tayebeh; Saidijam, Massoud; Aflatoonian, Reza; Amiri, Iraj; Ashrafi, Mahnaz; Shabab, Nooshin; Mohammadpour, Nooshin; Mehdizadeh, Mehdi
• Is Part Of: Gynecological endocrinology. Volume 31:Number 5(2015:May)
• Page Start: 379
• Page End: 383
• Publication Date: 2015-05
• Subjects: Endocrine gynecology -- Periodicals; Generative organs, Female -- Diseases -- Periodicals; 618.1
• DOI: 10.3109/09513590.2014.1001733
• Languages: English
• ISSNs: 0951-3590

Abstract

<abstract> <title>Abstract</title> <p>We evaluated gene expression of estrogen and progesterone nuclear receptors in granulosa cells (GCs) of polycystic ovary syndrome (PCOS) women compared to women with normal cycling ovaries (control group) to achieve a better understanding of ovarian steroid status in patients with PCOS. In this prospective study, 40 patients with PCOS and 40 women with normal ovulatory function who underwent <italic>in vitro</italic> fertilization (IVF) for treatment of tubal and/or male infertility were recruited. Follicular fluid was collected from patients and GCs were isolated from follicular fluid and then were purified with Micro Beads conjugated to monoclonal anti-human CD45 antibodies. RNA was extracted and reverse transcription was performed. Gene expression of estrogen and progesterone receptors was determined by quantitative real time PCR (qRT-PCR). Estrogen receptor β (ERβ) expression was significantly higher than ERα expression in both groups (<italic>p</italic> &lt; 0.002). ERα and ERβ mRNA expression in PCOS was significantly lower than control group (<italic>p</italic> &lt; 0.002). The expression levels of PRA and PRB in PCOS was significantly lower than control group (<italic>p</italic> &lt; 0.002). In conclusion, a significant reduction of these genes in GCs from follicles of women with PCOS could be considered as a sign for maturation defect or follicular arrest in GCs.</p> </abstract>