The influence of medium conductivity on ECIS measurements with field‐effect transistor arrays. Issue 6 (27th April 2015)

• Record Type: Journal Article
• Authors: Hempel, Felix; Nguyen, Thanh Chien; Law, Jessica Ka Yan; Ingebrandt, Sven
• Is Part Of: Physica status solidi. Volume 212:Issue 6(2015:Jun.)
• Page Start: 1260
• Page End: 1265
• Publication Date: 2015-04-27
• Subjects: Solid state physics -- Periodicals; Solids -- Industrial applications -- Periodicals; 530.41
• DOI: 10.1002/pssa.201431946
• Languages: English
• ISSNs: 1862-6300

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="pssa201431946-sec-0001" sec-type="section"> <p>Field‐effect transistors (FETs) offer a well‐known biosensor platform for many years now. In most of the applications, FETs are used as potentiometric sensors. In earlier studies, we used FETs for cell‐substrate impedance sensing and showed that in this way, the well‐known electric cell‐substrate impedance sensing (ECIS) method can be scaled down to a single cell resolution. When using new sensor types for ECIS, new coupling models for data interpretation are also needed. In this study, the influence of the conductivity of the measurement buffer and its effect on the recorded spectra was investigated. Human embryonic kidney cells were cultured on the FET surfaces and their impedance spectra were measured. By fitting the data to an established electrically equivalent circuit model, changes in the relevant cell parameters can be obtained. The derived cell‐related data offer an advanced understanding in cell transistor interface modeling.</p> </sec> </abstract>