Discriminating Lamiophlomis rotata According to Geographical Origin by 1H‐NMR Spectroscopy and Multivariate Analysis. Issue 4 (19th February 2015)

• Record Type: Journal Article
• Authors: Pan, Zheng; Fan, Gang; Yang, Rong‐ping; Luo, Wei‐zao; Zhou, Xiang‐dong; Zhang, Yi
• Is Part Of: Phytochemical analysis. Volume 26:Issue 4(2015:Jul.)
• Page Start: 247
• Page End: 252
• Publication Date: 2015-02-19
• Subjects: Plants -- Analysis -- Periodicals; Plants -- chemistry -- Periodicals; 572.2
• DOI: 10.1002/pca.2557
• Languages: English
• ISSNs: 0958-0344

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="pca2557-sec-0001" sec-type="section"> <title>Introduction</title> <p> <italic>Lamiophlomis rotata</italic> (Duyiwei) is a folk herbal medicine that traditionally has been used in China as a hemostatic agent. Raw plant materials used for medicinal products from different geographical regions are often inconsistent in chemical composition. Metabolic fingerprinting provides a new approach for distinguishing the geographical origins of <italic>L</italic>. <italic>rotata</italic>.</p> </sec> <sec id="pca2557-sec-0002" sec-type="section"> <title>Objective</title> <p>To identify metabolites that contribute to the different geographical regions of <italic>L</italic>. <italic>rotata</italic> samples.</p> </sec> <sec id="pca2557-sec-0003" sec-type="section"> <title>Methods</title> <p> <italic>Lamiophlomis rotata</italic> metabolomics were performed by <sup>1</sup>H‐nuclear magnetic resonance (NMR) spectroscopy and multivariate statistical analyses. The <italic>L</italic>. <italic>rotata</italic> metabolic profile was prepared for NMR measurements using methanol‐<italic>d<sub>4</sub></italic> solvent. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) were applied to analyse the <italic>L</italic>. <italic>rotata</italic><sup>1</sup>H‐NMR spectroscopy data.</p> </sec> <sec id="pca2557-sec-0004" sec-type="section"> <title>Results</title> <p>Nine iridoid glycosides, one flavonoid and three phenylpropanoid glycosides were detected in <italic>L</italic>. <italic>rotata</italic> by <sup>1</sup>H‐NMR spectroscopy. <sup>1</sup>H‐NMR measurements and multivariate analysis were used to successfully discriminate samples from three different locations.</p> </sec> <sec id="pca2557-sec-0005" sec-type="section"> <title>Conclusion</title> <p>The NMR‐based analysis of <italic>L</italic>. <italic>rotata</italic> is a more comprehensive approach than traditional chromatographic methods. Simple sample preparation, rapidity and reproducibility of are additional advantages of NMR analysis. Copyright © 2015 John Wiley & Sons, Ltd.</p> </sec> </abstract>