Alpinumisoflavone and abyssinone V 4′‐methylether derived from Erythrina lysistemon (Fabaceae) promote HDL‐cholesterol synthesis and prevent cholesterol gallstone formation in ovariectomized rats. (14th February 2015)

• Record Type: Journal Article
• Authors: Mvondo, Marie A.; Njamen, Dieudonné; Kretzschmar, Georg; Imma Bader, Manuela; Tanee Fomum, Stephen; Wandji, Jean; Vollmer, Günter
• Is Part Of: Journal of pharmacy and pharmacology. Volume 67:Number 7(2015:Jul.)
• Page Start: 990
• Page End: 996
• Publication Date: 2015-02-14
• Subjects: Pharmacy -- Periodicals; Pharmacology -- Periodicals; 615.1
• DOI: 10.1111/jphp.12386
• Languages: English
• ISSNs: 0022-3573

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="jphp12386-sec-0001" sec-type="section"> <title>Objectives</title> <p> <italic>E</italic> <italic>rythrina lysistemon</italic> was found to improve lipid profile in ovariectomized rats. Alpinumisoflavone (AIF) and abyssinone V 4′‐methylether (AME) derived from this plant induced analogous effects on lipid profile and decreased atherogenic risks. To highlight the molecular mechanism of action of these natural products, we evaluated their effects on the expression of some estrogen‐sensitive genes associated with cholesterol synthesis (<italic>Esr1</italic> and <italic>Apoa1</italic>) and cholesterol clearance (<italic>Ldlr</italic>, <italic>Scarb1</italic> and <italic>Cyp7a1</italic>).</p> </sec> <sec id="jphp12386-sec-0002" sec-type="section"> <title>Methods</title> <p>Ovariectomized rats were subcutaneously treated for three consecutive days with either compound at the daily dose of 0.1, 1 and 10 mg/kg body weight (BW). Animals were sacrificed thereafter and their liver was collected. The mRNA of genes of interest was analysed by quantitative real‐time polymerase chain reaction.</p> </sec> <sec id="jphp12386-sec-0003" sec-type="section"> <title>Key findings</title> <p>Both compounds downregulated the mRNA expression of <italic>Esr1</italic>, a gene associated with cholesterogenesis and cholesterol gallstone formation. AME leaned the <italic>Apoa1/Scarb1</italic> balance in favour of <italic>Apoa1</italic>, an effect promoting high‐density lipoprotein (HDL)‐cholesterol formation. It also upregulated the mRNA expression of <italic>Ldlr</italic> at 1 mg/kg/BW per day (25%) and 10 mg/kg/BW per day (133.17%), an effect favouring the clearance of low‐density lipoprotein (LDL)‐cholesterol. Both compounds may also promote the conversion of cholesterol into bile acids as they upregulated <italic>Cyp7a1</italic> mRNA expression.</p> </sec> <sec id="jphp12386-sec-0004" sec-type="section"> <title>Conclusion</title> <p>AIF and AME atheroprotective effects may result from their ability to upregulate mechanisms promoting HDL‐cholesterol and bile acid formation.</p> </sec> </abstract>