Histomolecular interpretation of pleomorphic adenomas of the salivary gland by matrix‐assisted laser desorption ionization imaging and spatial segmentation. Issue 7 (11th July 2014)

• Record Type: Journal Article
• Authors: Ernst, Günther; Guntinas–Lichius, Orlando; Hauberg–Lotte, Lena; Trede, Dennis; Becker, Michael; Alexandrov, Theodore; von Eggeling, Ferdinand
• Is Part Of: Head & neck. Volume 37:Issue 7(2015:Jul.)
• Page Start: 1014
• Page End: 1021
• Publication Date: 2014-07-11
• Subjects: Head -- Diseases -- Periodicals; Neck -- Diseases -- Periodicals; Head -- Periodicals; Neck -- Periodicals; Face -- Periodicals; 617.51059
• DOI: 10.1002/hed.23713
• Languages: English
• ISSNs: 1043-3074

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="hed23713-sec-0001" sec-type="section"> <title>Background</title> <p>Despite efforts in localization of key proteins using immunohistochemistry, the complex proteomic composition of pleomorphic adenomas has not yet been characterized. Matrix‐assisted laser desorption/ionization imaging mass spectrometry (MALDI imaging) allows label‐free and spatially resolved detection of hundreds of proteins directly from tissue sections and of histomorphological regions by finding colocalized molecular signals. Spatial segmentation of MALDI imaging data is an algorithmic method for finding regions of similar proteomic composition as functionally similar regions.</p> </sec> <sec id="hed23713-sec-0002" sec-type="section"> <title>Methods</title> <p>We investigated 2 pleomorphic adenomas by applying spatial segmentation to the MALDI imaging data of tissue sections.</p> </sec> <sec id="hed23713-sec-0003" sec-type="section"> <title>Results</title> <p>The spatial segmentation subdivided the tissue in a good accordance with the tissue histology. Numerous molecular signals colocalized with histologically defined tissue regions were found.</p> </sec> <sec id="hed23713-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Our study highlights the cellular transdifferentiation within the pleomorphic adenoma. It could be shown that spatial segmentation of MALDI imaging data is a promising approach in the emerging field of digital histological analysis and characterization of tumors. © 2014 Wiley Periodicals, Inc. Head Neck 37: 1014–1021, 2015</p> </sec> </abstract>