Epigenetic alterations in metastatic cutaneous carcinoma. Issue 7 (27th June 2014)

• Record Type: Journal Article
• Authors: Darr, Owen A.; Colacino, Justin A.; Tang, Alice L.; McHugh, Jonathan B.; Bellile, Emily L.; Bradford, Carol R.; Prince, Mark P.; Chepeha, Douglas B.; Rozek, Laura S.; Moyer, Jeffrey S.
• Is Part Of: Head & neck. Volume 37:Issue 7(2015:Jul.)
• Page Start: 994
• Page End: 1001
• Publication Date: 2014-06-27
• Subjects: Head -- Diseases -- Periodicals; Neck -- Diseases -- Periodicals; Head -- Periodicals; Neck -- Periodicals; Face -- Periodicals; 617.51059
• DOI: 10.1002/hed.23701
• Languages: English
• ISSNs: 1043-3074

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <sec id="hed23701-sec-0001" sec-type="section"> <title>Background</title> <p>Squamous cell carcinoma (SCC) and basal cell carcinoma (BCC) are the 2 most common cutaneous carcinomas. Molecular profiles predicting metastasis of these cancers have not been identified.</p> </sec> <sec id="hed23701-sec-0002" sec-type="section"> <title>Methods</title> <p>Epigenetic profiles of 37 primary cases of cutaneous SCC and BCC were quantified via the Illumina Goldengate Cancer Panel. Differential protein expression by metastatic potential was analyzed in 110 total cases by immunohistochemical (IHC) staining.</p> </sec> <sec id="hed23701-sec-0003" sec-type="section"> <title>Results</title> <p>Unsupervised hierarchical clustering analysis revealed that metastatic BCCs had a methylation profile resembling cutaneous SCCs. Metastatic cutaneous SCCs were found to be hypermethylated at <italic>FRZB</italic> (median methylation: 46.7% vs 4.7%; <italic>p</italic> = 4 × 10<sup>−5</sup>). Metastatic BCCs were found to be hypomethylated at <italic>MYCL2</italic> (median methylation: 3.8% vs 83.4%; <italic>p</italic> = 1.9 × 10<sup>−6</sup>). Immunohistochemical staining revealed few differences between metastatic and nonmetastatic cancers.</p> </sec> <sec id="hed23701-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Metastatic primary BCCs and cutaneous SCCs had distinct epigenetic profiles when compared to their nonmetastatic counterparts. Epigenetic profiling may prove useful in future diagnosis and prevention of advanced nonmelanoma skin cancers. © 2014 Wiley Periodicals, Inc. Head Neck 37: 994–1001, 2015</p> </sec> </abstract>