Functional patterns of cytomegalovirus (CMV) pp65 and immediate early‐1‐specific CD8+ T cells that are associated with protection from and control of CMV DNAemia after allogeneic stem cell transplantation. Issue 3 (1st June 2015)

• Record Type: Journal Article
• Authors: Giménez, E.; Muñoz‐Cobo, B.; Solano, C.; Amat, P.; de la Cámara, R.; Nieto, J.; López, J.; Remigia, M.J.; Garcia‐Noblejas, A.; Navarro, D.
• Is Part Of: Transplant infectious disease. Volume 17:Issue 3(2015)
• Page Start: 361
• Page End: 370
• Publication Date: 2015-06-01
• Subjects: Transplantation of organs, tissues, etc -- Complications -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; 617.01
• DOI: 10.1111/tid.12391
• Languages: English
• ISSNs: 1398-2273

Abstract

<abstract abstract-type="main" id="tid12391-abs-0001"> <title>Abstract</title> <sec id="tid12391-sec-0001" sec-type="section"> <title>Background</title> <p>The functional profile of cytomegalovirus (CMV)‐specific CD8<sup>+</sup> T cells that associate with protection from and control of CMV DNAemia in allogeneic stem cell transplant (allo‐SCT) recipients remains incompletely characterized.</p> </sec> <sec id="tid12391-sec-0002" sec-type="section"> <title>Methods</title> <p>We enumerated pp65 and immediate early (IE)‐1‐specific CD8<sup>+</sup> T cells expressing interferon‐gamma, tumor necrosis factor‐alpha, and CD107a, by flow cytometry in 94 patients at days +30 and +60 after allo‐SCT.</p> </sec> <sec id="tid12391-sec-0003" sec-type="section"> <title>Results</title> <p>Fifty of 94 patients had CMV DNAemia within the first 100 days after transplant. CMV‐specific CD8<sup>+</sup> T‐cell responses (of any functional type) were more likely to be detected in patients who did not display CMV DNAemia than in those who did (<italic>P</italic> = 0.04). Qualitatively, no major differences in the functional signature of CMV‐specific CD8<sup>+</sup> T cells were noted between patients who had or did not have CMV DNAemia. Patients displaying levels of polyfunctional CD8<sup>+</sup> T cells at day +30 >0.30 cell/μL had a lower risk of CMV DNAemia (positive predictive value 76%, and negative predictive value 43%).</p> </sec> <sec id="tid12391-sec-0004" sec-type="section"> <title>Conclusion</title> <p>The presence of polyfunctional CD8<sup>+</sup> T cells (either expressing CD107a or not) was associated with lower levels of CMV replication, and higher frequency of self‐resolved episodes. The data reported further clarify the role of polyfunctional CD8<sup>+</sup> T cells in control of CMV DNAemia in allo‐SCT recipients.</p> </sec> </abstract>