Comparative Epidemiology of Streptococcus pyogenes emm-Types Causing Invasive and Noninvasive Infections in French Children by Use of High-Resolution Melting-Polymerase Chain Reaction. Issue 6 (June 2015)

• Record Type: Journal Article
• Authors: d'Humières, Camille; Bidet, Philippe; Levy, Corinne; Béchet, Stéphane; Bonacorsi, Stéphane; Bingen, Edouard; Cohen, Robert
• Is Part Of: Pediatric infectious disease journal. Volume 34:Issue 6(2015)
• Publication Date: 2015-06
• Subjects: Communicable diseases in children -- Periodicals; Infection in children -- Periodicals; 618.929
• DOI: 10.1097/INF.0000000000000677
• Languages: English
• ISSNs: 0891-3668

Abstract

<abstract> <title> <x xml:space="preserve">Abstract</x> </title> <sec> <title>Background:</title> <p>This study aims to analyze the epidemiology of Group A streptococci (GAS) <italic>emm</italic>-types causing invasive and noninvasive infections in French children.</p> </sec> <sec> <title>Methods:</title> <p>From September 2009 to May 2011, we analyzed GAS isolates from 585 pharyngitis, 125 invasive infections and, for the first time in France, 32 healthy carriers. M protein gene (<italic>emm</italic>) typing of the isolates was carried out by a new rapid technique, combining 3 multiplex-polymerase chain reactions (PCRs) coupled to high-resolution melting (HRM) curves, able to detect 13 major <italic>emm</italic>-types (<italic>emm</italic> 1, 3, 4, 6, 11, 12, 22, 28, 75, 77, 87, 89 and 102).</p> </sec> <sec> <title>Results:</title> <p>GAS belonging to <italic>emm</italic>-type 1 were more frequently found among invasive infections than among pharyngitis (24.0% vs. 11.5%, <italic>P</italic> < 0.001); <italic>emm</italic> 4 and 89 were more common in pharyngitis than in invasive infections (<italic>emm</italic>-type 4, 17.4% vs. 6.4%, <italic>P</italic> = 0.002 and <italic>emm</italic>-type 89, 9.9% vs. 2.4%, <italic>P</italic> = 0.006, respectively) and <italic>emm</italic> 3 and 4 were more common in cases of pharyngitis associated with scarlet fever (21.6% vs. 6.0%, <italic>P</italic> < 0.001 and 29.3% vs. 14.5%, <italic>P</italic> < 0.001, respectively).</p> </sec> <sec> <title>Conclusion:</title> <p>HRM method enables the rapid <italic>emm</italic>-typing of a large number of isolates in epidemiological studies. Comparison of GAS causing invasive and noninvasive infections in the same population of children displays an unbalanced repartition of <italic>emm</italic>-types.</p> </sec> </abstract>