Nasopharyngeal and Adenoid Colonization by Haemophilus influenzae and Haemophilus parainfluenzae in Children Undergoing Adenoidectomy and the Ability of Bacterial Isolates to Biofilm Production. Issue 18 (May 2015)

• Record Type: Journal Article
• Authors: Kosikowska, Urszula; Korona-Głowniak, Izabela; Niedzielski, Artur; Malm, Anna; Dessein., Rodrigue
• Is Part Of: Medicine. Volume 94:Issue 18(2015)
• Publication Date: 2015-05
• Subjects: Medicine -- Periodicals; Medicine -- Periodicals; Médecine -- Périodiques; Geneeskunde; Medicine; Periodicals; Periodicals; 610.5
• DOI: 10.1097/MD.0000000000000799
• Languages: English
• ISSNs: 0025-7974

Abstract

<abstract> <title> <x xml:space="preserve">Abstract</x> </title> <sec> <title>Abstract</title> <p>Haemophili are pathogenic or opportunistic bacteria often colonizing the upper respiratory tract mucosa. The prevalence of <italic>Haemophilus influenzae</italic> (with serotypes distribution), and <italic>H. parainfluenzae</italic> in the nasopharynx and/or the adenoid core in children with recurrent pharyngotonsillitis undergoing adenoidectomy was assessed. Haemophili isolates were investigated for their ability to biofilm production.</p> <p>Nasopharyngeal swabs and the adenoid core were collected from 164 children who underwent adenoidectomy (2–5 years old). Bacteria were identified by the standard methods. Serotyping of <italic>H. influenzae</italic> was performed using polyclonal and monoclonal antisera. Biofilm formation was detected spectrophotometrically using 96-well microplates and 0.1% crystal violet.</p> <p>Ninety seven percent (159/164) children who underwent adenoidectomy were colonized by <italic>Haemophilus</italic> spp. The adenoid core was colonized in 99.4% (158/159) children, whereas the nasopharynx in 47.2% (75/159) children (<italic>P</italic> < 0.0001). In 32% (51/159) children only encapsulated (typeable) isolates of <italic>H. influenzae</italic> were identified, in 22.6% (36/159) children only (nonencapsulated) <italic>H. influenzae</italic> NTHi (nonencapsulated) isolates were present, whereas 7.5% (12/159) children were colonized by both types. 14.5% (23/159) children were colonized by untypeable (rough) <italic>H. influenzae</italic>. In 22% (35/159) children <italic>H. influenzae</italic> serotype d was isolated. Totally, 192 isolates of <italic>H. influenzae</italic>, 96 isolates of <italic>H. parainfluenzae</italic> and 14 isolates of other <italic>Haemophilus</italic> spp. were selected. In 20.1% (32/159) children 2 or 3 phenotypically different isolates of the same species (<italic>H. influenzae</italic> or <italic>H. parainfluenzae</italic>) or serotypes (<italic>H</italic>. <italic>influenzae</italic>) were identified in 1 child. 67.2% (129/192) isolates of <italic>H</italic>. <italic>influenzae, </italic> 56.3% (54/96) isolates of <italic>H. parainfluenzae</italic> and 85.7% (12/14) isolates of other <italic>Haemophilus</italic> spp. were positive for biofilm production. Statistically significant differences (<italic>P</italic> = 0.0029) among <italic>H. parainfluenzae</italic> biofilmproducers and nonproducers in the adenoid core and the nasopharynx were detected.</p> <p> <italic>H</italic>. <italic>influenzae</italic> and <italic>H</italic>. <italic>parainfluenzae</italic> carriage rate was comparatively higher in the adenoid core than that in the nasopharynx in children undergoing adenoidectomy, suggesting that their involvement in chronic adenoiditis. The growth in the biofilm seems to be an important feature of haemophili colonizing the upper respiratory tract responsible for their persistence.</p> </sec> </abstract>