Decreased Programmed Death-1 Expression on the T Cells of Patients With Ankylosing Spondylitis. (June 2015)

• Record Type: Journal Article
• Authors: Zhou, Lianlian; Zhang, Yanhong; Xu, Huiying; Hu, Liping; Zhang, Chunwu; Sun, Li; Xie, Yaosheng; Lu, Hong; Zhang, Zhuo; Hu, Wangqiang; Lin, Xiangyang
• Is Part Of: American journal of the medical sciences. Volume 349:Number 6(2015)
• Publication Date: 2015-06
• Subjects: Medicine -- Periodicals; Internal medicine -- Periodicals; 610.5
• DOI: 10.1097/MAJ.0000000000000468
• Languages: English
• ISSNs: 0002-9629

Abstract

<abstract> <title>Abstract:</title> <sec> <title>Background:</title> <p>Programmed death-1 (PD-1) plays a vital role in down-modulating immune responses and maintaining peripheral tolerance.</p> </sec> <sec> <title>Methods:</title> <p>The authors have investigated the inducible expression of PD-1 on activated T cells from patients with ankylosing spondylitis (AS). Thirty patients with AS and 31 unrelated healthy controls (HCs) were recruited in this study. The expression of PD-1 on T cells harvested from nonstimulated (t<sub>0</sub>) or stimulated cultures with phytohemagglutinin for 24 hours (t<sub>24</sub>) was determined by flow cytometry. The multiple levels of the PD-1 expression on stimulated and nonstimulated cells from each individual's sample (t<sub>24</sub>/t<sub>0</sub>) represented as the degree of the inducible effect on PD-1 expression.</p> </sec> <sec> <title>Results:</title> <p>The expression of PD-1 on nonstimulated T cells presented no significant difference between AS group and HC group (<italic>P</italic> > 0.05). After stimulation, the degree of effect on PD-1 expression of CD4<sup>+</sup>, CD4<sup>+</sup>CD25<sup>+</sup>, CD4<sup>+</sup>CD25<sup>high</sup>, CD4<sup>+</sup>CD25<sup>low</sup> and CD4<sup>+</sup>CD25<sup>−</sup> T cells were significantly lower in patients with AS than those in HC group (1.9 ± 0.9 versus 3.6 ± 2.3, 9.7 ± 7.4 versus 17.8 ± 12.6, 87.8 ± 48.6 versus 157.3 ± 117.0, 3.7 ± 1.4 versus 7.3 ± 2.4, 0.5 ± 0.3 versus 1.1 ± 0.6, respectively, <italic>P</italic> < 0.05). However, there was no significant difference of the effect on all lymphocytes and CD8<sup>+</sup> T cells between patients with AS and HCs (<italic>P</italic> > 0.05).</p> </sec> <sec> <title>Conclusions:</title> <p>The decreased inducible expression of PD-1 on active T lymphocytes, especially on CD4<sup>+</sup>CD25<sup>high</sup> and CD4<sup>+</sup>CD25<sup>+</sup> T cells, may be one of important factors involved in the development of AS.</p> </sec> </abstract>