Vitrification of sperm from marine fish: effect on motility and membrane integrity. (6th December 2013)

• Record Type: Journal Article
• Authors: Cuevas‐Uribe, Rafael; Chesney, Edward J; Daly, Jonathan; Tiersch, Terrence R
• Is Part Of: Aquaculture research. Volume 46:Number 7(2015)
• Page Start: 1770
• Page End: 1784
• Publication Date: 2013-12-06
• Subjects: Aquaculture -- Periodicals; Fishery management -- Periodicals; 639.8
• DOI: 10.1111/are.12337
• Languages: English
• ISSNs: 1355-557X

Abstract

<abstract abstract-type="main" id="are12337-abs-0001"> <title>Abstract</title> <p>Our goal was to develop a standardized approach for sperm vitrification of marine fish that can be applied generally in aquatic species. The objectives were to: (i) estimate acute toxicity of cryoprotectants over a range of concentrations; (ii) evaluate the properties of vitrification solutions (VS); (iii) evaluate different thawing solutions and (iv) evaluate sperm quality after thawing by examination of motility and membrane integrity. Sperm were collected from red snapper (<italic>Lutjanus campechanus</italic>), spotted seatrout (<italic>Cynoscion nebulosus</italic>) and red drum (<italic>Sciaenops ocellatus</italic>). A total of 29 combinations of cryoprotectants were evaluated for toxicity and glass formation. Samples were loaded onto 10‐μL polystyrene loops and plunged into liquid nitrogen. There was a significant difference (<italic>P</italic> < 0.05) in post‐thaw motility among VS and among species when using the same VS. The sperm in VS of 15% DMSO + 15% ethylene glycol + 10% glycerol + 1% X‐1000<sup>™</sup> + 1% Z‐1000<sup>™</sup> had an average post‐thaw motility of 58% and membrane integrity of 19% for spotted seatrout, 38% and 9% for red snapper, and 30% and 19% for red drum. Adaptations by marine fish to higher osmotic pressures could explain the survival in the high cryoprotectant concentrations. Vitrification offers an alternative to conventional cryopreservation.</p> </abstract>