Sequence variability in four mitochondrial genes among rabbit pinworm (Passalurus ambiguus) isolates from different localities in China. (August 2015)

• Record Type: Journal Article
• Authors: Sheng, Li; Cui, Ping; Fang, Su-Fang; Lin, Rui-Qing; Zou, Feng-Cai; Zhu, Xing-Quan
• Is Part Of: Mitochondrial DNA. Volume 26:Number 4(2015:Aug.)
• Page Start: 501
• Page End: 504
• Publication Date: 2015-08
• Subjects: Mitochondrial DNA -- Periodicals; Mitochondrial DNA -- Abnormalities -- Periodicals; Nucleotide sequence -- Periodicals; Gene mapping -- Periodicals; 572.869
• DOI: 10.3109/19401736.2013.855898
• Languages: English
• ISSNs: 1940-1736

Abstract

<abstract> <title>Abstract</title> <p> <italic>Passalurus ambiguus</italic> is a common pinworm which parasitizes in the caecum and colon of rabbits. This study examined genetic variability among <italic>P. ambiguus</italic> isolated from naturally infected rabbits in four different provinces in China. The partial mitochondrial (mt) cytochrome <italic>c</italic> oxidase subunit 1 (p<italic>cox</italic>1), cytochrome <italic>b</italic> (p<italic>cytb</italic>) and NADH dehydrogenase subunits 1 and 5 (p<italic>nad</italic>1 and p<italic>nad</italic>5) were amplified separately from individual nematodes by PCR and sequenced. The results showed that p<italic>cox</italic>1, p<italic>cyt</italic>b, p<italic>nad</italic>1 and p<italic>nad</italic>5 were 714, 663, 645 and 546 bp in length, respectively. The intra-specific sequence variations within <italic>P. ambiguus</italic> were 0–1.1% for p<italic>cox</italic>1, 0–1.2% for p<italic>cyt</italic>b, 0–0.6% for p<italic>nad</italic>1 and 0–1.3% for p<italic>nad</italic>5, whereas inter-specific sequence differences with other members of the Oxyuridae were 16.2–17.3% for p<italic>cox</italic>1, 27.8–30.4% for p<italic>cyt</italic>b, 20.2–24.0% for p<italic>nad</italic>1 and 27.1–30.3% for p<italic>nad</italic>5. Phylogenetic analyses using Bayesian inference (BI), maximum likelihood (ML) and maximum parsimony (MP) methods, based on the combined sequences of the four partial mtDNA sequences, revealed that all the <italic>P. ambiguus</italic> samples form monophyletic groups. This study demonstrated the existence of low-level intra-specific variation in <italic>cox</italic>1, <italic>cyt</italic>b, <italic>nad</italic>1 and <italic>nad</italic>5 genes among <italic>P. ambiguus</italic> isolates from different geographic regions in China, and these four mtDNA sequences can be used as genetic markers for the population genetic studies of <italic>P. ambiguus</italic>, as well as the differentiation of <italic>P. ambiguus</italic> from other oxyurid nematodes.</p> </abstract>