Genetic characterization of group A acetylsaponin‐deficient mutants from wild soybean (Glycine soja Sieb. and Zucc.). Issue 3 (21st April 2015)

• Record Type: Journal Article
• Authors: Krishnamurthy, Panneerselvam; Lee, Jeong‐Dong; Ha, Bo‐Keun; Chae, Jong‐Hyun; Song, Jong Tae; Tsukamoto, Chigen; Singh, Ram J.; Chung, Gyuhwa; Link, W.
• Is Part Of: Plant breeding. Volume 134:Issue 3(2015)
• Page Start: 316
• Page End: 321
• Publication Date: 2015-04-21
• Subjects: Plant breeding -- Periodicals; 631.52
• DOI: 10.1111/pbr.12269
• Languages: English
• ISSNs: 0179-9541

Abstract

<abstract abstract-type="main" id="pbr12269-abs-0001"> <title>Abstract</title> <p>Group A acetylsaponins are the main causative components for bitter and astringent tastes of soybean (<italic>Glycine max</italic>). In this study, we examined the genetic nature of the absence of group A acetylsaponins in 12 Korean wild soybean (<italic>Glycine soja</italic>) accessions. In all 12 accessions, the coding region (1431‐bp) of <italic>Sg‐1</italic> locus was identical with <italic>Sg‐1</italic>^{<italic>a</italic>}, which adds the xylose sugar moiety at the terminal position of the C‐22 sugar chain of SS‐A, except one nucleotide (G→A change) at +948th position. This point mutation results in change of one amino acid from tryptophan (TGG) to stop codon (TGA). We observed that the mutated <italic>Sg‐1</italic> was controlled by a single recessive gene (<italic>sg‐1</italic>^{<italic>0‐a1</italic>}). This gene was mapped between BARCSOYSSR_07_1561 and BARCSOYSSR_07_1598 on soybean chromosome 7. Our study demonstrated that the mutated <italic>Sg‐1</italic> gene in Korean wild soybeans is genetically different from those identified in Japanese soybean cultivar 'Kinusayaka' and wild soybean JP‐36121. We believe that the new <italic>Sg‐1</italic> mutants can also be utilized to produce a new soybean variety without bitter and astringent properties.</p> </abstract>