Effect of high temperature on the production of 2n pollen grains in diploid roses and obtaining tetraploids via unilateral polyploidization. Issue 3 (11th May 2015)

• Record Type: Journal Article
• Authors: Crespel, Laurent; Le Bras, Camille; Relion, Daniel; Roman, Hanaé; Morel, Philippe; Debener, T.
• Is Part Of: Plant breeding. Volume 134:Issue 3(2015)
• Page Start: 356
• Page End: 364
• Publication Date: 2015-05-11
• Subjects: Plant breeding -- Periodicals; 631.52
• DOI: 10.1111/pbr.12271
• Languages: English
• ISSNs: 0179-9541

Abstract

<abstract abstract-type="main" id="pbr12271-abs-0001"> <title>Abstract</title> <p>To integrate the gene pool of a wild species (primarily diploid) into a cultivated pool (primarily tetraploid), a crossing between a dihaploid cultivated rose and a hybrid of <italic>Rosa wichurana</italic> allowed to obtain interspecific diploid hybrids that produced 2n pollen grains. A return to a tetraploid level sought by breeders can then be considered using sexual polyploidization, obtained by crossing a tetraploid cultivated rose with these hybrids. Application of a high‐temperature regime led to a small but significant increase in the percentage of 2n pollen grains in these hybrids of up to 4.6%. This result was obtained by applying high temperatures close to 32°C during the day to plants cultivated in a glasshouse during recurrent cycles of bloom. Crosses were made between an unreleased tetraploid hybrid tea rose, as a female, and the diploid hybrid that produces the most 2n pollen grains. Tetraploid (42.1%) and triploid (57.9%) offspring were obtained. The use of these 2n pollen grains of the first division restitution type should facilitate the introgression of complex traits of interest.</p> </abstract>