Fine mapping of qSS‐9, a major and stable quantitative trait locus, for seed storability in rice (Oryza sativa L.). Issue 3 (8th April 2015)

• Record Type: Journal Article
• Authors: Lin, Qiuyun; Jiang, Yimei; Sun, Ailing; Cao, Penghui; Li, Linfang; Liu, Xi; Tian, Yunlu; He, Jun; Liu, Shijia; Chen, Liangming; Jiang, Ling; Ahn, S.N.
• Is Part Of: Plant breeding. Volume 134:Issue 3(2015)
• Page Start: 293
• Page End: 299
• Publication Date: 2015-04-08
• Subjects: Plant breeding -- Periodicals; 631.52
• DOI: 10.1111/pbr.12264
• Languages: English
• ISSNs: 0179-9541

Abstract

<abstract abstract-type="main" id="pbr12264-abs-0001"> <title>Abstract</title> <p>Seed storability in rice (<italic>Oryza sativa</italic> L.) is an important agronomic trait. We previously showed a quantitative trait locus of seed storability, <italic>qSS‐9</italic>, on chromosome 9 in a backcross population of 'Koshihikari' (<italic>japonica</italic>) / 'Kasalath' (<italic>indica</italic>) // 'Koshihikari'. In this study, fine mapping of the chromosomal location of <italic>qSS‐9</italic> was performed. Effect of 'Kasalath' allele of <italic>qSS‐9</italic> was validated using a chromosome segment substitution line, SL36, which harboured the target quantitative trait loci (QTL) from 'Kasalath' in the genetic background of 'Nipponbare' under different ageing treatments in different environments. Subsequently, an F₂ population from a cross between 'Nipponbare' and SL36 was used for fine mapping of <italic>qSS‐9</italic>. Simultaneously, four subnear isogenic lines (sub‐NILs) that represented different recombination breakpoints across the <italic>qSS‐9</italic> region were developed from F₃ progeny. Finally, the <italic>qSS‐9</italic> locus was located between the Indel markers Y10 and Y13, which delimit a region of 147 kb in the 'Nipponbare' genome. These results provide a springboard for map‐based cloning of <italic>qSS‐9</italic> and possibilities for breeding rice varieties with strong seed storability.</p> </abstract>