Basic fibroblastic growth factor affects the osteogenic differentiation of dental pulp stem cells in a treatment‐dependent manner. (11th September 2014)

• Record Type: Journal Article
• Authors: Qian, J.; Jiayuan, W.; Wenkai, J.; Peina, W.; Ansheng, Z.; Shukai, S.; Shafei, Z.; Jun, L.; Longxing, N.
• Is Part Of: International endontic journal. Volume 48:Number 7(2015:Jul.)
• Page Start: 690
• Page End: 700
• Publication Date: 2014-09-11
• Subjects: Endodontics -- Periodicals; 617.6342
• DOI: 10.1111/iej.12368
• Languages: English
• ISSNs: 0143-2885

Abstract

<abstract abstract-type="main" id="iej12368-abs-0001"> <title>Abstract</title> <sec id="iej12368-sec-0001" sec-type="section"> <title>Aim</title> <p>To determine how basic fibroblastic growth factor (bFGF) affected the osteogenic differentiation of human dental pulp stem cells (DPSCs) <italic>in vitro</italic> and <italic>in vivo</italic>.</p> </sec> <sec id="iej12368-sec-0002" sec-type="section"> <title>Methodology</title> <p>Basic fibroblastic growth factor stimulation of DPSCs was divided into a pre‐treatment period and an osteogenic differentiation period. Alizarin red quantification experiments and alkaline phosphatase activity quantification assay were performed to examine the osteogenic differentiation of DPSCs after different bFGF stimulation. Quantification reverse transcription polymerase chain reaction was used to analyze the osteogenic gene expression of DPSCs after different bFGF stimulation. In addition, DPSCs that received the 1 and 2 weeks bFGF pre‐treatments as in the <italic>in vitro</italic> experiments were mineralized for 1 week and seeded into hydroxyapatite/tricalcium phosphate (HA/TCP) pills and subcutaneously transplanted into naked mice for 2 or 3 months. The transplants were removed, sliced and stained using Modified Ponceau Trichrome Stain to observe the formation of mineralized tissue.</p> </sec> <sec id="iej12368-sec-0003" sec-type="section"> <title>Results</title> <p>Basic fibroblastic growth factor stimulation in the osteogenic differentiation period decreased the <italic>in vitro</italic> osteogenic differentiation ability of DPSCs. One week pre‐treatment with bFGF increased the <italic>in vitro</italic> osteogenic differentiation ability of DPSCs, whereas 2 weeks pre‐treatment with bFGF decreased the <italic>in vitro</italic> osteogenic differentiation ability of DPSCs. The pre‐treatment period was vital for the osteogenic differentiation of DPSCs <italic>in vitro</italic>. The <italic>in vivo</italic> results were similar to the <italic>in vitro</italic> results.</p> </sec> <sec id="iej12368-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Basic fibroblastic growth factor affected the osteogenic differentiation of DPSCs in a treatment‐dependent manner both <italic>in vitro</italic> and <italic>in vivo</italic>.</p> </sec> </abstract>