Rat precision-cut intestinal slices to study P-gp activity and the potency of its inhibitors ex vivo. Issue 5 (August 2015)

• Record Type: Journal Article
• Authors: Li, Ming; de Graaf, Inge A.M.; de Jager, Marina H.; Groothuis, Geny M.M.
• Is Part Of: Toxicology in vitro. Volume 29:Issue 5(2015)
• Page Start: 1070
• Page End: 1078
• Publication Date: 2015-08
• Subjects: Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2015.04.011
• Languages: English
• ISSNs: 0887-2333

Abstract

<abstract xml:lang="en" abstract-type="author-highlights" id="ab005"> <title id="st005">Highlights</title> <sec> <p id="sp0005"> <list id="l0005"> <list-item id="o0005"> <label></label> <p id="p0005">P-gp inhibitors can be identified using Precision-Cut Intestinal Slices (PCIS).</p> </list-item> <list-item id="o0010"> <label></label> <p id="p0010">Relative potency (IC₅₀) of P-gp inhibitors is correctly predicted in PCIS.</p> </list-item> <list-item id="o0015"> <label></label> <p id="p0015">P-gp activity in rat is higher in ileum than in duodenum, jejunum and colon.</p> </list-item> <list-item id="o0020"> <label></label> <p id="p0020">P-gp inhibition increases accumulation of Rhodamine 123 in the enterocytes of PCIS.</p> </list-item> </list> </p> </sec> </abstract>