Lysosomal Targeting of Cystinosin Requires AP‐3. (24th March 2015)

• Record Type: Journal Article
• Authors: Andrzejewska, Zuzanna; Névo, Nathalie; Thomas, Lucie; Bailleux, Anne; Chauvet, Véronique; Benmerah, Alexandre; Antignac, Corinne
• Is Part Of: Traffic. Volume 16:Number 7(2015:Jul.)
• Page Start: 712
• Page End: 726
• Publication Date: 2015-03-24
• Subjects: Biological transport -- Periodicals; 571.6
• DOI: 10.1111/tra.12277
• Languages: English
• ISSNs: 1398-9219

Abstract

<abstract abstract-type="graphical" id="tra12277-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p id="tra12277-para-0001">Cystinosin, a seven transmembrane (TM) lysosomal cystine transporter defective in the autosomal recessive lysosomal storage disorder, has been shown to be targeted to lysosomes via two sorting signals: a tyrosine‐based motif in its C‐terminal (GYDQL) and a non‐classical motif in its fifth inter‐TM loop. Here, we show that the tyrosine‐based motif specifically interacts with AP‐3 complex and functions as a 'strong' AP‐3 motif responsible for direct intracellular targeting of cystinosin and the CD63‐cystinosin chimera (CD63‐GYDQL) to late endosomes/lysosomes.<graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgjrgxbtcf" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></p> </abstract>