Nerium indicum leaf alleviates iron-induced oxidative stress and hepatic injury in mice. (July 2015)

• Record Type: Journal Article
• Authors: Ghate, Nikhil Baban; Chaudhuri, Dipankar; Panja, Sourav; Mandal, Nripendranath
• Is Part Of: Pharmaceutical biology. Volume 53:Number 7(2015:Jul.)
• Page Start: 1066
• Page End: 1074
• Publication Date: 2015-07
• Subjects: Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2014.959612
• Languages: English
• ISSNs: 1388-0209

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context</italic>: <italic>Nerium indicum</italic> Mill. (Apocynaceae) was reported for its efficient <italic>in vitro</italic> antioxidant and iron-chelating properties.</p> <p> <italic>Objective</italic>: This study demonstrates the effect of 70% methanol extract of <italic>N. indicum</italic> leaf (NIME) towards <italic>in vitro</italic> DNA protection and ameliorating iron-overload-induced liver damage in mice.</p> <p> <italic>Materials and methods</italic>: Phytochemical and HPLC analyses were carried out to standardize the extract and the effect of Fe<sup>2+</sup>-mediated pUC18 DNA cessation was studied. Thirty-six Swiss Albino mice were divided into six groups of blank, negative control (iron overload only), and iron-overloaded mice receiving 50, 100, and 200 mg/kg b.w. doses of NIME and desirox (20 mg/kg b.w.). The biochemical markers of hepatic damage, various liver and serum parameters, and reductive release of ferritin iron were studied.</p> <p> <italic>Results and discussion</italic>: The presence of different phytocomponents was revealed from phytochemical and HPLC analyses. A substantial supercoiled DNA protection, with [<italic>P</italic>]<sub>50</sub> of 70.33 ± 0.32 µg, was observed. NIME (200 mg/kg b.w.) significantly normalized the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and bilirubin by 126.27, 125.25, 188.48, and 45.47%, respectively. NIME (200 mg/kg b.w.) was shown to alleviate the reduced levels of superoxide dismutase, catalase, glutathione-<italic>S</italic>-transferase, and non-enzymatic-reduced glutathione, by 48.95, 35.9, 35.42, and 13.22%, respectively. NIME also lowered raised levels of lipid peroxidation, protein carbonyl, hydroxyproline, and liver iron by 32.28, 64.58, 136.81, and 83.55%, respectively.</p> <p> <italic>Conclusion</italic>: These findings suggest that the active substances present in NIME may be capable of lessening iron overload-induced toxicity, and possibly be a useful drug for iron-overloaded diseases.</p> </abstract>