Down-regulation of the CYP19A1 gene in cumulus cells of infertile women with endometriosis. Issue 5 (May 2015)

• Record Type: Journal Article
• Authors: Barcelos, Ionara Diniz E.S.; Donabella, Flávia Capello; Ribas, Cristiana Padovan; Meola, Juliana; Ferriani, Rui Alberto; de Paz, Cláudia Cristina Paro; Navarro, Paula A.
• Is Part Of: Reproductive biomedicine online. Volume 30:Issue 5(2015)
• Page Start: 532
• Page End: 541
• Publication Date: 2015-05
• Subjects: Human reproductive technology -- Periodicals; Human embryo -- Periodicals; Reproduction -- Periodicals; 616.692
• DOI: 10.1016/j.rbmo.2015.01.012
• Languages: English
• ISSNs: 1472-6483

Abstract

<abstract abstract-type="author" id="ab0010"> <title id="st0010">Abstract</title> <sec> <p id="sp0010">Aromatase plays a fundamental role in the establishment of oocyte quality, which might be compromised in infertile women with endometriosis. The expression of the <italic>CYP19A1</italic> gene (that encodes aromatase) was compared in cumulus cells and oestradiol concentrations in the follicular fluid of infertile women with and without endometriosis submitted to ovarian stimulation for intracytoplasmic sperm injection. Cumulus cells were isolated and the expression of the <italic>CYP19A1</italic> was quantitated through real-time polymerase chain reaction. Oestradiol concentrations in follicular fluid were measured by chemiluminescence immunoassay. A lower expression of the <italic>CYP19A1</italic> in the cumulus cells of infertile women with endometriosis was observed compared with controls (0.17 ± 0.13 and 0.56 ± 0.12, respectively), and no significant difference in the follicular fluid oestradiol concentrations was observed between groups. Our results show reduced expression of the <italic>CYP19A1</italic> in cumulus cells of infertile women with endometriosis, which may play a role in the pathogenesis of endometriosis-related infertility.</p> </sec> </abstract>