Detection of knockdown resistance mutations in the common bed bug, Cimex lectularius (Hemiptera: Cimicidae), in Australia. Issue 7 (21st August 2014)

• Record Type: Journal Article
• Authors: Dang, Kai; Toi, Cheryl S; Lilly, David G; Bu, Wenjun; Doggett, Stephen L
• Is Part Of: Pest management science. Volume 71:Issue 7(2015:Jul.)
• Page Start: 914
• Page End: 922
• Publication Date: 2014-08-21
• Subjects: Pests -- Control -- Periodicals; Pesticides -- Periodicals; 632.9
• DOI: 10.1002/ps.3861
• Languages: English
• ISSNs: 1526-498X

Abstract

<abstract abstract-type="main" id="ps3861-abs-0001"> <title>Abstract</title> <sec id="ps3861-sec-0001" sec-type="section"> <title>BACKGROUND</title> <p id="ps3861-para-0001">Pyrethroid resistance in the common bed bug, <italic>Cimex lectularius</italic> L., has been reported worldwide. An important resistance mechanism is via knockdown resistance (<italic>kdr</italic>) mutations, notably V419L and L925I. Information regarding this <italic>kdr</italic>‐type resistance mechanism is unknown in Australia. This study aims to examine the status of <italic>kdr</italic> mutations in Australian <italic>C. lectularius</italic> strains.</p> </sec> <sec id="ps3861-sec-0002" sec-type="section"> <title>RESULTS</title> <p id="ps3861-para-0002">Several modern field‐collected strains and museum‐preserved reference collections of Australian <italic>C. lectularius</italic> were examined. Of the field strains (2007–2013), 96% had the known <italic>kdr</italic> mutations (L925I or both V419L/L925I). The 'Adelaide' strain (2013) and samples from the preserved reference collections (1994–2002) revealed no known <italic>kdr</italic> mutations. A novel mutation I936F was apparent in the insecticide‐resistant 'Adelaide' strain, one strain from Perth (with L925I) and the majority of the reference collection specimens. The laboratory insecticide‐resistant 'Sydney' strain showed a mixture of no <italic>kdr</italic> mutations (20%) and L925I (80%).</p> </sec> <sec id="ps3861-sec-0003" sec-type="section"> <title>CONCLUSION</title> <p id="ps3861-para-0003">The novel mutation I936F may be a <italic>kdr</italic> mutation but appeared to contribute less resistance to the pyrethroids than the V419L and L925I mutations. The detection of high frequencies of <italic>kdr</italic> mutations indicates that <italic>kdr</italic>‐type resistance is widespread across Australia. Hence, there should be a reduced reliance on pyrethroid insecticides and an integrated management approach for the control of <italic>C. lectularius</italic> infestations. © 2014 Society of Chemical Industry</p> </sec> </abstract>