Age‐associated decrease of senescence marker protein‐30/gluconolactonase in individual mouse liver cells: Immunohistochemistry and immunofluorescence. Issue 6 (14th October 2014)

• Record Type: Journal Article
• Authors: Ishigami, Akihito; Masutomi, Hirofumi; Handa, Setsuko; Maruyama, Naoki
• Is Part Of: Geriatrics and gerontology international. Volume 15:Issue 6(2015)
• Page Start: 804
• Page End: 810
• Publication Date: 2014-10-14
• Subjects: Geriatrics -- Periodicals; Gerontology -- Periodicals; Geriatrics -- Japan -- Periodicals; Gerontology -- Japan -- Periodicals; 618.97
• DOI: 10.1111/ggi.12347
• Languages: English
• ISSNs: 1444-1586

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="ggi12347-sec-0001" sec-type="section"> <title>Aim</title> <p>Senescence marker protein‐30 (SMP30)/gluconolactonase (GNL) is an age‐associated protein in that its presence decreases with aging. Here, we used immunohistochemical analysis to investigate the changes of SMP30/GNL in individual cells of the liver from progressively aged mice.</p> </sec> <sec id="ggi12347-sec-0002" sec-type="section"> <title>Methods</title> <p>Male C57BL/6 strain mice at 1, 3, 6, 12, 24 and 30 months‐of‐age were the source of hepatic cells used to detect SMP30/GNL. Liver sections from these mice were subjected to immunohistochemical staining with anti‐SMP30/GNL antibody. For immunofluorescent staining, primary cultured hepatocytes from mice at various ages were stained with SMP30/GNL and albumin.</p> </sec> <sec id="ggi12347-sec-0003" sec-type="section"> <title>Results</title> <p>In liver cells from mice of all ages, SMP30/GNL staining appeared in some but not all parenchymal cells, and localized in both the nuclei and cytoplasm. Moreover, SMP30/GNL‐positive staining of parenchymal cells was present only around central vein areas, but not at sites of portal veins. Furthermore, the number of SMP30/GNL‐positive cells increased as mice aged from 1 to 12 months, then decreased from the 12th to 24th month. Results were similar in primary cultured hepatocytes from mice of various ages.</p> </sec> <sec id="ggi12347-sec-0004" sec-type="section"> <title>Conclusions</title> <p>SMP30/GNL‐positive cells localized mainly around the central veins in the livers of mice and decreased numerically with aging, although there was no age‐related change in counts of albumin‐positive cells. SMP30/GNL protein occupied the nuclei and cytoplasm. Therefore, nuclear SMP30/GNL protein might be a regulatory factor specific for genes whose expression governs transcription and the aging process. <bold>Geriatr Gerontol Int 2015; 15: 804–810.</bold></p> </sec> </abstract>